Time course of collagen peak in bile duct-ligated rats

BMC Gastroenterology

Nurdan Tözün⁶  Cigdem Celikel⁹  Gilbert Hilman⁷  Ned Snyder¹  Gagan Sood¹  Nevin Yilmaz⁴  Ilker Sücüllü²  Gülgün Tahan⁷  Veysel Tahan⁵  Metin Basaranoglu³  Orhan Tarcin⁸ 

[1] University of Texas Medical Branch, Division of Gastroenterology, Galveston/USA;Gülhane Military Medical Faculty, Haydarpasa Educational Hospital Istanbul, 34500, Turkey;Division of Gastroenterology and Hepatology, Consulting, Ankara Yüksek Ihtisas Hospital Gastroenterology Clinic, Sihhiye, Ankara, 06420, Turkey;University of Texas Medical Branch, Division of Pharmacology, Galveston/USA;Institute of Gastroenterology, Surgery Unit, Marmara University Istanbul, 34500, Turkey;Division of Gastroenterology Acıbadem University, Istanbul, 34500, Turkey;Division of Gastroenterology, Marmara University Istanbul, 34500, Turkey;Acibadem Hospital, Istanbul, 34500, Turkey;Division of Pathology Marmara University, Istanbul, 34500, Turkey

关键词: reversible;    collagen;    liver;    fibrogenesis;    fibrosis;    rat;    Bile duct-ligated;   

Others  :  1114554
DOI  :  10.1186/1471-230X-11-45

 received in 2010-10-16, accepted in 2011-04-28,  发布年份 2011

【 摘 要 】

Background

One of the most useful experimental fibrogenesis models is the "bile duct-ligated rats". Our aim was to investigate the quantitative hepatic collagen content by two different methods during the different stages of hepatic fibrosis in bile duct-ligated rats on a weekly basis. We questioned whether the 1-wk or 4-wk bile duct-ligated model is suitable in animal fibrogenesis trials.

Methods

Of the 53 male Wistar rats, 8 (Group 0) were used as a healthy control group. Bile duct ligation (BDL) had been performed in the rest. Bile duct-ligated rates were sacrificed 7 days later in group 1 (10 rats), 14 days later in group 2 (9 rats), 21 days later in group 3(9 rats) and 28 days later in group 4 (9 rats). Eight rats underwent sham-operation (Sham). Hepatic collagen measurements as well as serum levels of liver enzymes and function tests were all analysed.

Results

The peak level of collagen was observed biochemically and histomorphometricly at the end of third week (P < 0.001 and P < 0.05). Suprisingly, collagen levels had decreased with the course of time such as at the end of fourth week (P < 0.01 and P < 0.05).

Conclusion

We have shown that fibrosis in bile duct-ligated rats is transient, i.e. reverses spontaneously after 3 weeks. This contrasts any situation in patients where hepatic fibrosis is progressive and irreversible as countless studies performed by many investigators in the same animal model.

【 授权许可】

   
2011 Tarcin et al; licensee BioMed Central Ltd.

【 图 表 】

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