Pretreatment of parecoxib attenuates hepatic ischemia/reperfusion injury in rats

BMC Anesthesiology

Research Article

Wen-Qi Huang¹  Kang-Qing Xu¹  Tao Zhang¹  Yi Ma² 

[1] Department of Anesthesiology, the First Affiliated Hospital, Sun Yat-sen University, 510080, Guangzhou, China;Organ transplantation center, the First Affiliated Hospital, Sun Yat-sen University, 510080, Guangzhou, China;

关键词: Hepatic ischemia/reperfusion;    Parecoxib;    Cyclooxygenase-2;    Inflammation;   

DOI  :  10.1186/s12871-015-0147-0

 received in 2015-05-05, accepted in 2015-11-11,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPrevious studies showed that cyclooxygenase(COX) was involved in ischemia/reperfusion (I/R) injuries. Parecoxib, a selective inhibitor for COX −2, has been shown to have protective properties in reducing I/R injury in the heart, kidney and brain. The aim of this study was to investigate the effects of parecoxib on hepatic I/R and to explore the underlying mechanisms.MethodsFifty-two Sprague–Dawley rats were randomly divided into three groups: the sham-operation (Sham) group, the hepatic ischemia/reperfusion (I/R) group, and the parecoxib pretreated I/R (I/R + Pare) group. Partial warm ischemia was produced in the left and middle hepatic lobes of Sprague–Dawley rats for 60 min, followed by 6 h of reperfusion. Rats in the I/R + Pare group received parecoxib (10 mg/kg) intraperitoneally twice a day for three consecutive days prior to ischemia. Blood and tissue samples from the groups were collected 6 h after reperfusion, and a survival study was performed.ResultsPretreatment with parecoxib prior to I/R insult significantly reduced I/R-induced elevations of aminotransferases, and significantly improved the histological status of the liver. Parecoxib significantly suppressed inflammatory cascades, as demonstrated by attenuations in TNF-α and IL-6. Parecoxib significantly inhibited iNOS and nitrotyrosine expression after I/R and significantly attenuated I/R-induced apoptosis. The 7-day survival rate was increased by pre-administration of parecoxib.ConclusionsAdministration of parecoxib prior to hepatic I/R attenuates hepatic injury through inhibition of inflammatory response and nitrosative stress.

【 授权许可】

CC BY   
© Zhang et al. 2015