Exercise training restores the cardiac microRNA-1 and −214 levels regulating Ca2+ handling after myocardial infarction

BMC Cardiovascular Disorders

Research Article

Diego Robles Mazzotti¹  Lucienne da Silva Lara²  Tiago Fernandes³  Vander José Neves³  Stéphano Freitas Soares Melo³  Edilamar Menezes Oliveira³  Valério Garrone Barauna⁴ 

[1] Department of Health Informatics, Federal University of São Paulo, Sao Paulo, Brazil;Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;Laboratory of Biochemistry and Molecular Biology of the Exercise, School of Physical Education and Sport, University of Sao Paulo, Av. Professor Mello Moraes, 65- Cidade Universitária, Sao Paulo, Brazil;Laboratory of Molecular Physiology, Health Sciences Center, Federal University of Espírito Santo, Vitória, Brazil;

关键词: Exercise training;    Myocardial infarction;    MicroRNA;    Cardiac function;   

DOI  :  10.1186/s12872-015-0156-4

 received in 2015-07-07, accepted in 2015-11-20,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundImpaired cardiomyocyte contractility and calcium handling are hallmarks of left ventricular contractile dysfunction. Exercise training has been used as a remarkable strategy in the treatment of heart disease. The microRNA-1, which targets sodium/calcium exchanger 1 (NCX), and microRNA-214, which targets sarcoplasmic reticulum calcium ATPase-2a (Serca2a), are involved in cardiac function regulation. Thus, the aim of this study was to evaluate the effect of exercise training on cardiac microRNA-1 and −214 expression after myocardial infarction.MethodsWistar rats were randomized into four groups: sedentary sham (S-SHAM), sedentary infarction (S-INF), trained sham (T-SHAM), and trained infarction (T-INF). Exercise training consisted of 60 min/days, 5 days/week for 10 weeks with 3 % of body weight as overload beginning four weeks after myocardial infarction.ResultsMicroRNA-1 and −214 expressions were, respectively, decreased (52 %) and increased (54 %) in the S-INF compared to the S-SHAM, while exercise training normalized the expression of these microRNAs. The microRNA targets NCX and Serca-2a protein expression were, respectively, decreased (55 %) and increased (34 %) in the T-INF group compared to the S-INF group.ConclusionsThese results suggest that exercise training restores microRNA-1 and −214 expression levels and prevents change in both NCX and Serca-2a protein and gene expressions. Altogether, our data suggest a molecular mechanism to restore ventricular function after exercise training in myocardial infarction rats.

【 授权许可】

CC BY   
© Melo et al. 2015