Serum lipidomic profiling as a useful tool for screening potential biomarkers of hepatitis B-related hepatocellular carcinoma by ultraperformance liquid chromatography–mass spectrometry

BMC Cancer

Research Article

João Renato Rebello Pinho¹  Aline Lopes Chagas¹  Fernanda Malta¹  Luciana Kikuchi¹  Michele Soares Gomes-Gouvêa¹  Flair José Carrilho¹  Ana Catharina de Seixas-Santos Nastri²  Ana Maria Passos-Castilho³  Celso Francisco Hernandes Granato⁴  Valdemir Melechco Carvalho⁵  Karina Helena Morais Cardozo⁵ 

[1] Department of Gastroenterology, Sao Paulo Clinicas Liver Cancer Group, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Sao Paulo, SP, Brazil;Department of Infectious Diseases, University of São Paulo School of Medicine, Sao Paulo, SP, Brazil;Division of Infectious Diseases, Federal University of Sao Paulo, 781 Pedro de Toledo Street, 15th floor, 04039032, Sao Paulo, SP, Brazil;Division of Infectious Diseases, Federal University of Sao Paulo, 781 Pedro de Toledo Street, 15th floor, 04039032, Sao Paulo, SP, Brazil;Fleury Group, Sao Paulo, SP, Brazil;Fleury Group, Sao Paulo, SP, Brazil;

关键词: Biomarker;    Hepatocellular carcinoma;    Hepatitis B;    Lipidomics;    UPLC-MS;    Diagnosis;   

DOI  :  10.1186/s12885-015-1995-1

 received in 2015-07-03, accepted in 2015-12-10,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundChronic hepatitis B (CHB) virus infection is a major cause of hepatocellular carcinoma (HCC), as late diagnosis is the main factor for the poor survival of patients. There is an urgent need for accurate biomarkers for early diagnosis of HCC. The aim of the study was to explore the serum lipidome profiles of hepatitis B-related HCC to identify potential diagnostic biomarkers.MethodsAn ultraperformance liquid chromatography mass spectrometry (UPLC-MS) lipidomic method was used to characterize serum profiles from HCC (n = 32), liver cirrhosis (LC) (n = 30), CHB (n = 25), and healthy subjects (n = 34). Patients were diagnosed by clinical laboratory and imaging evidence and all presented with CHB while healthy controls had normal liver function and no infectious diseases.ResultsThe UPLC-MS-based serum lipidomic profile provided more accurate diagnosis for LC patients than conventional alpha-fetoprotein (AFP) detection. HCC patients were discriminated from LC with 78 % sensitivity and 64 % specificity. In comparison, AFP showed sensitivity and specificity of 38 % and 93 %, respectively. HCC was differentiated from CHB with 100 % sensitivity and specificity using the UPLC-MS approach. Identified lipids comprised glycerophosphocolines, glycerophosphoserines and glycerophosphoinositols.ConclusionsUPLC-MS lipid profiling proved to be an efficient and convenient tool for diagnosis and screening of HCC in a high-risk population.

【 授权许可】

CC BY   
© Passos-Castilho et al. 2015