Angelica sinensis extract inhibits RANKL-mediated osteoclastogenesis by down-regulated the expression of NFATc1 in mouse bone marrow cells

BMC Complementary and Alternative Medicine

Research Article

Lingbo Kong¹  Xiaodong Wang¹  Qinpeng Zhao¹  Dingjun Hao¹  Chongfei Yang²  Jinyu Zhu² 

[1] Hong-Hui Hospital, Xi’an Jiaotong University College of Medicine, 710054, Xi’an, China;Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, 710054, Xi’an, China;

关键词: Angelica sinensis;    Osteoclastogenesis;    NFATc1;    BMMs;   

DOI  :  10.1186/1472-6882-14-481

 received in 2014-05-14, accepted in 2014-12-10,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDestructive erosion of bone or osteolysis is a major complication of inflammatory conditions such as rheumatoid arthritis (RA), periodontal disease, and periprosthetic osteolysis. Natural plant-derived products have received recent attention as potential therapeutic and preventative drugs in human disease.MethodsThe effect of Angelica sinensis (AS) extract on RANKL-induced osteoclast differentiation was examined in this study. The osteoclast precursor cell line bone marrow macrophages (BMMs) was cultured and stimulated with RANKL followed by treatment with AS at several doses. Gene expression profiles of c-Fos, c-Jun, NFATc1, TRAP, and OSCAR were sequentially evaluated.ResultsAS extract inhibited RANKL-mediated osteoclast differentiation in BMMs in a dose-dependent manner without any evidence of cytotoxicity. AS extract strongly inhibited p38, ERK, JNK, p65 phosphorylation and I-κB degradation in RANKL-stimulated BMMs. AS extract also inhibited the mRNA expression of c-Fos, c-Jun, NFATc1, TRAP, and OSCAR in RANKL-treated BMMs. Moreover, RANKL-induced c-Fos, c-Jun and NFATc1 protein expression was suppressed by AS extract.ConclusionsThese results collectively suggested that AS extract demonstrated inhibitory effects on RANKL-mediated osteoclast differentiation in bone marrow macrophages in vitro, indicating that AS may therefore serve as a useful drug in the prevention of bone loss.

【 授权许可】

Unknown   
© Kong et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.