Vellozia flavicans Mart. ex Schult. hydroalcoholic extract inhibits the neuromuscular blockade induced by Bothrops jararacussu venom

BMC Complementary and Alternative Medicine

Research Article

Marcio Galdino dos Santos¹  Eliana Aparecida Varanda²  Magali Glauzer Silva³  Alexandro Mateus da Silva³  Miriéle Cristina Ferraz³  Natália Tribuiani³  Yoko Oshima-Franco³  Francisco Carlos Groppo⁴  Talita Signoreti Graziano⁴  Ana Paula Guerreiro Bentes⁴  Karina Cogo⁵  José Carlos Cogo⁶ 

[1] Environmental Sciences post-graduation program, PGCiamb, Tocantins Federal University (UFT), Av NS 15 ALC NO 14, 109 Norte, CEP 77001-090, Porto Nacional, Tocantins, Brazil;Pharmaceutical Sciences Faculty of Araraquara, São Paulo State University (UNESP), CEP 14801-902, Rodovia Araraquara-Jau, Km 1, Araraquara, São Paulo, Brazil;Pharmaceutical Sciences post-graduation program, University of Sorocaba (UNISO), CEP 18023-000, Rodovia Raposo Tavares km 92.5, Sorocaba, SP, Brazil;Piracicaba Dental School – University of Campinas (UNICAMP), CEP 13414-903, Av. Limeira 901, Piracicaba, SP, Brazil;Piracicaba Dental School – University of Campinas (UNICAMP), CEP 13414-903, Av. Limeira 901, Piracicaba, SP, Brazil;Dentistry Department, de Santo Amaro University (UNISA), CEP 04829-300, R. Prof. Eneas de Siqueira Neto 340, Santo Amaro, SP, Brazil;Serpentárium of the Vale do Paraíba University (CEN - UNIVAP), CEP 12244-000, Av Shishima Hifumi 2911, São José dos Campos, SP, Brazil;

关键词: Antimicrobial;    Antiophidian;    Medicinal plant;    Snake venom;   

DOI  :  10.1186/1472-6882-14-48

 received in 2013-05-08, accepted in 2014-02-04,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundSnakebite is a significant public health issue in tropical countries. In Brazil, some of the most common snake envenomations are from Bothrops. Bothrops bites trigger local and systemic effects including edema, pain, erythema, cyanosis, infections, and necrosis. Vellozia flavicans is a plant from the Brazilian “cerrado” (savanna) that is popularly used as an anti-inflammatory medicine. Since inflammation develops quickly after Bothrops bites, which can lead to infection, the aim of the present study was to observe possible anti-snake venom and antimicrobial activities of V. flavicans (Vf).MethodsThe chromatographic profile of the main constituents from the Vf leaf hydroalcoholic extract was obtained by thin-layer chromatography (TLC). The anti-snake venom activity was measured by Vf’s ability to neutralize the in vitro neuromuscular blockade caused by Bothrops jararacussu venom (Bjssu) in a mouse phrenic nerve-diaphragm model (PND). After a 20 min incubation, preparations of PND were added to Tyrode’s solution (control); Vf (0.2, 0.5, 1, and 2 mg/mL); 40 μg/mL Bjssu; pre-incubation for 30 min with Bjssu and 1 mg/mL Vf; and a Bjssu pretreated preparation (for 10 min) followed by 1 mg/mL Vf. Myographic recording was performed, and the contractile responses were recorded. The antimicrobial activity (minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC]) was obtained for Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis, using gentamicin and vancomycin as positive controls.ResultsTLC analysis yielded several compounds from Vf, such as flavonoids (quercetin) and phenolic acids (chlorogenic acid). Bjssu completely blocked the contractile responses of PND preparations, while Vf preserved 97% (±10%) of the contractile responses when incubated with Bjssu. In the PND pretreated with Bjssu, Vf was able to inhibit the neuromuscular blockade progress. MIC and MBC of Vf ranged from 2.5 to 5.0 mg/mL for P. aeruginosa and S. aureus strains, while no antimicrobial activity was observed for E. coli and E. faecalis.ConclusionsThe hydroalcoholic extract from Vf leaves was able to neutralize and decrease the in vitro neuromuscular blockade caused by Bjssu. However, it did not show significant antimicrobial activity against the tested bacteria.

【 授权许可】

Unknown   
© Tribuiani et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.