Preventive effects of Chlorella on skeletal muscle atrophy in muscle-specific mitochondrial aldehyde dehydrogenase 2 activity-deficient mice

BMC Complementary and Alternative Medicine

Research Article

Ikuroh Ohsawa¹  Yoshihiko Suzuki²  Kiyomi Nishimaki²  Shigeo Ohta²  Isao Maruyama³  Shoichiro Kumamoto³  Yuya Nakashima⁴ 

[1] Biological Process of Aging, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae, 173-0015, Itabashi-ku, Tokyo, Japan;Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Nippon Medical School, 1-396 Kosugi-machi, 211-8533, Nakahara-ku, Kawasaki, Kanagawa, Japan;Department of Research and Development, Chlorella Industry Co. Ltd, 1343 Hisatomi, 833-0056, Chikugo, Fukuoka, Japan;Department of Research and Development, Chlorella Industry Co. Ltd, 1343 Hisatomi, 833-0056, Chikugo, Fukuoka, Japan;Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Nippon Medical School, 1-396 Kosugi-machi, 211-8533, Nakahara-ku, Kawasaki, Kanagawa, Japan;

关键词: Muscle atrophy;    Chlorella;    Aldehyde dehydrogenase 2;    c;    Oxidative stress;   

DOI  :  10.1186/1472-6882-14-390

 received in 2014-05-23, accepted in 2014-10-07,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundOxidative stress is involved in age-related muscle atrophy, such as sarcopenia. Since Chlorella, a unicellular green alga, contains various antioxidant substances, we used a mouse model of enhanced oxidative stress to investigate whether Chlorella could prevent muscle atrophy.MethodsAldehyde dehydrogenase 2 (ALDH2) is an anti-oxidative enzyme that detoxifies reactive aldehydes derived from lipid peroxides such as 4-hydroxy-2-nonenal (4-HNE). We therefore used transgenic mice expressing a dominant-negative form of ALDH2 (ALDH2*2 Tg mice) to selectively decrease ALDH2 activity in the muscles. To evaluate the effect of Chlorella, the mice were fed a Chlorella-supplemented diet (CSD) for 6 months.ResultsALDH2*2 Tg mice exhibited small body size, muscle atrophy, decreased fat content, osteopenia, and kyphosis, accompanied by increased muscular 4-HNE levels. The CSD helped in recovery of body weight, enhanced oxidative stress, and increased levels of a muscle impairment marker, creatine phosphokinase (CPK) induced by ALDH2*2. Furthermore, histological and histochemical analyses revealed that the consumption of the CSD improved skeletal muscle atrophy and the activity of the mitochondrial cytochrome c oxidase.ConclusionsThis study suggests that long-term consumption of Chlorella has the potential to prevent age-related muscle atrophy.

【 授权许可】

CC BY   
© Nakashima et al.; licensee BioMed Central Ltd. 2014