Preventive effects of Chlorella on skeletal muscle atrophy in muscle-specific mitochondrial aldehyde dehydrogenase 2 activity-deficient mice

BMC Complementary and Alternative Medicine

Shigeo Ohta³  Yoshihiko Suzuki³  Isao Maruyama²  Shoichiro Kumamoto²  Kiyomi Nishimaki³  Ikuroh Ohsawa¹  Yuya Nakashima³ 

[1] Biological Process of Aging, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae, Itabashi-ku, Tokyo 173-0015, Japan;Department of Research and Development, Chlorella Industry Co. Ltd, 1343 Hisatomi, Chikugo, Fukuoka 833-0056, Japan;Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Nippon Medical School, 1-396 Kosugi-machi, Nakahara-ku, Kawasaki, Kanagawa 211-8533, Japan

关键词: Oxidative stress;    Mitochondrial cytochrome c oxidase;    Aldehyde dehydrogenase 2;    Chlorella;    Muscle atrophy;   

Others  :  1086121
DOI  :  10.1186/1472-6882-14-390

 received in 2014-05-23, accepted in 2014-10-07,  发布年份 2014

【 摘 要 】

Background

Oxidative stress is involved in age-related muscle atrophy, such as sarcopenia. Since Chlorella, a unicellular green alga, contains various antioxidant substances, we used a mouse model of enhanced oxidative stress to investigate whether Chlorella could prevent muscle atrophy.

Methods

Aldehyde dehydrogenase 2 (ALDH2) is an anti-oxidative enzyme that detoxifies reactive aldehydes derived from lipid peroxides such as 4-hydroxy-2-nonenal (4-HNE). We therefore used transgenic mice expressing a dominant-negative form of ALDH2 (ALDH2*2 Tg mice) to selectively decrease ALDH2 activity in the muscles. To evaluate the effect of Chlorella, the mice were fed a Chlorella-supplemented diet (CSD) for 6 months.

Results

ALDH2*2 Tg mice exhibited small body size, muscle atrophy, decreased fat content, osteopenia, and kyphosis, accompanied by increased muscular 4-HNE levels. The CSD helped in recovery of body weight, enhanced oxidative stress, and increased levels of a muscle impairment marker, creatine phosphokinase (CPK) induced by ALDH2*2. Furthermore, histological and histochemical analyses revealed that the consumption of the CSD improved skeletal muscle atrophy and the activity of the mitochondrial cytochrome c oxidase.

Conclusions

This study suggests that long-term consumption of Chlorella has the potential to prevent age-related muscle atrophy.

【 授权许可】

   
2014 Nakashima et al.; licensee BioMed Central Ltd.

【 图 表 】

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