Nigella sativa fixed and essential oil modulates glutathione redox enzymes in potassium bromate induced oxidative stress

BMC Complementary and Alternative Medicine

Research Article

Saikat Dewanjee¹  Shakeel Ahmad²  Hawa ZE Jaafar³  Roselina Karim⁴  Ubedullah Kaka⁵  Muhammad Tauseef Sultan⁶  Waqas Ahmed⁷  Masood Sadiq Butt⁷  M. Zia-Ul-Haq⁸ 

[1] Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, 700032, Kolkata, India;Department of Agronomy, Bahauddin Zakariya University Multan, Multan, Pakistan;Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, Serdang, Selangor, Malaysia;Faculty of Food Science & Technology, University of Putra Malaysia, Serdang, Selangor, MY, Malaysia;Faculty of Veterinary Medicines, University of Putra Malaysia, Serdang, Selangor, Malaysia;Institute of Food Science and Nutrition, Bahauddin Zakariya University, Multan, Pakistan;National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan;The Patent Office, Karachi, Pakistan;

关键词: Nigella sativa;    Functional foods;    Oxidative stress;    Glutathione;    Myeloperoxidase;   

DOI  :  10.1186/s12906-015-0853-7

 received in 2015-04-05, accepted in 2015-09-07,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundNigella sativa is an important component of several traditional herbal preparations in various countries. It finds its applications in improving overall health and boosting immunity. The current study evaluated the role of fixed and essential oil of Nigella sativa against potassium bromate induced oxidative stress with special reference to modulation of glutathione redox enzymes and myeloperoxidase.MethodsAnimals; 30 rats (Sprague Dawley) were divided in three groups and oxidative stress was induced using mild dose of potassium bromate. The groups were on their respective diets (iso-caloric diets for a period of 56 days) i.e. control and two experimental diets containing N. sativa fixed (4 %) and essential (0.3 %) oils. The activities of enzymes involved in glutathione redox system and myeloperoxidase (MPO) were analyzed.ResultsThe experimental diets modulated the activities of enzymes i.e. glutathione-S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx) positively. Indices of antioxidant status like tocopherols and glutathione were in linear relationship with that of GPx, GR and GST (P < 0.01). MPO activities were in negative correlation with GST (P < 0.01) but positive correlation with some other parameters.ConclusionsOur results indicated that both Nigella sativa fixed and essential oil are effective in improving the antioxidant indices against potassium bromate induced oxidative stress.

【 授权许可】

CC BY   
© Sultan et al. 2015