Nigella sativa fixed and essential oil modulates glutathione redox enzymes in potassium bromate induced oxidative stress

BMC Complementary and Alternative Medicine

M. Zia-Ul-Haq¹  Hawa ZE Jaafar⁸  Saikat Dewanjee³  Shakeel Ahmad⁵  Ubedullah Kaka⁴  Waqas Ahmed⁶  Roselina Karim⁷  Masood Sadiq Butt⁶  Muhammad Tauseef Sultan² 

[1] The Patent Office, Karachi, Pakistan;Institute of Food Science and Nutrition, Bahauddin Zakariya University, Multan, Pakistan;Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India;Faculty of Veterinary Medicines, University of Putra Malaysia, Serdang, Selangor, Malaysia;Department of Agronomy, Bahauddin Zakariya University Multan, Multan, Pakistan;National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan;Faculty of Food Science & Technology, University of Putra Malaysia, Serdang, Selangor, MY, Malaysia;Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, Serdang, Selangor, Malaysia

关键词: Myeloperoxidase;    Glutathione;    Oxidative stress;    Functional foods;    Nigella sativa;   

Others  :  1226043
DOI  :  10.1186/s12906-015-0853-7

 received in 2015-04-05, accepted in 2015-09-07,  发布年份 2015

【 摘 要 】

Background

Nigella sativa is an important component of several traditional herbal preparations in various countries. It finds its applications in improving overall health and boosting immunity. The current study evaluated the role of fixed and essential oil of Nigella sativa against potassium bromate induced oxidative stress with special reference to modulation of glutathione redox enzymes and myeloperoxidase.

Methods

Animals; 30 rats (Sprague Dawley) were divided in three groups and oxidative stress was induced using mild dose of potassium bromate. The groups were on their respective diets (iso-caloric diets for a period of 56 days) i.e. control and two experimental diets containing N. sativa fixed (4 %) and essential (0.3 %) oils. The activities of enzymes involved in glutathione redox system and myeloperoxidase (MPO) were analyzed.

Results

The experimental diets modulated the activities of enzymes i.e. glutathione-S-transferase (GST), glutathione reductase (GR) and glutathione peroxidase (GPx) positively. Indices of antioxidant status like tocopherols and glutathione were in linear relationship with that of GPx, GR and GST (P < 0.01). MPO activities were in negative correlation with GST (P < 0.01) but positive correlation with some other parameters.

Conclusions

Our results indicated that both Nigella sativa fixed and essential oil are effective in improving the antioxidant indices against potassium bromate induced oxidative stress.

【 授权许可】

   
2015 Sultan et al.

【 参考文献 】

• [1]Sultan MT, Butt MS, Qayyum MM, Suleria HA. Immunity: plants as effective mediators. Crit Rev Food Sci Nutr. 2014; 54(10):1298-308.
• [2]Andersen CJ, Fernandez ML. Dietary strategies to reduce metabolic syndrome. Rev Endocr Metab Disord. 2013; 14:241-54.
• [3]Sultan MT, Butt MS, Ahmad RS, Pasha I, Ahmad AN, Qayyum MM. Supplementation of Nigella sativa fixed and essential oil mediates potassium bromate induced oxidative stress and multiple organ toxicity. Pak J Pharm Sci. 2012; 25:175-181.
• [4]Butt MS, Sultan MT. Nigella sativa reduces the risk of various maladies. Crit Rev Food Sci Nutr. 2010; 50:654-665.
• [5]Shabana A, El-Menyar A, Asim M, Al-Azzeh H, Al TH. Cardiovascular benefits of Black Cumin (Nigella sativa). Cardiovasc Toxicol. 2013; 13:9-21.
• [6]Zia-Ul-Haq M, Ahmad S, Bukhari SA, Amarowicz R, Ercisli S, Jaafar HZE. Compositional studies and biological activities of some mash bean (Vigna mungo (L.) Hepper) cultivars commonly consumed in Pakistan. Biol Res. 2014; 47:23-31. BioMed Central Full Text
• [7]Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Anwar F. A review on therapeutic potential of Nigella sativa: a miracle herb. Asian Pac J Trop Biomed. 2013; 3:337-352.
• [8]Sultan MT, Butt MS, Anjum FM. Safety assessment of Nigella sativa fixed and essential oils in normal Sprague Dawley: serological and hematological indices. Food Chem Toxicol. 2009; 47:2768-2775.
• [9]Uchida K, Satoh T, Ogura Y, Yamaga N, Yamada K. Effect of partial ileal bypass on cholesterol and bile acid metabolism in rats. Yanago Acta Medica. 2001; 44:69-77.
• [10]Ohkawa N, Ohishi J, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95:351-358.
• [11]Hermes-Lima M, Willmore WG, Storey KB. Quantification of lipid peroxidation in tissue extracts based on Fe(III)xylenol orange complex formation. Free Radic Biol Med. 1995; 19:271-280.
• [12]Corongiu FP, Milla A. An improved method for determining diene conjugation in autoxidized polyunsaturated fatty acids. Chem Biol Interact. 1983; 44:289-297.
• [13]Beutler E. A manual of biochemical methods. Grune and Stratton Publisher, New-York, USA; 1982.
• [14]Xu Z, Godber JS. Purification and identification of components of gamma-oryzanol in rice bran oil. J Agric Food Chem. 1999; 47:2724-2728.
• [15]Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988; 34:497.
• [16]Block PP, Karmer R, Paverka M. A simple assay for catalase determination. Cell Biol Monog. 1980; 7:44-47.
• [17]Tappel AL. Glutathione peroxidase and hydroperoxides. Method Enzym. 1978; 52:506-513.
• [18]Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70:158-169.
• [19]Hillegas LM, Griswold DE, Brickson B, Albrightson-Winslow C. Assessment of myeloperoxidase activity in whole rat kidney. J Pharmacol Meth. 1990; 24:285-295.
• [20]Singh AS, Pal DT, Mandal BC, Singh P, Pathak NN. Studies on changes in some of blood constituents of adult cross-bred cattle fed different levels of extracted rice bran. Pak J Nutr. 2002; 1:95-98.
• [21]Morita O, Tamaki Y, Kirkpatrick JB, Chengelis CP. Safety assessment of heated diacylglycerol oil: Subchronic toxicity study in rats. Food Chem Toxicol. 2008; 46:2748-2757.
• [22]Wajs A, Bonikowski R, Kalemba D. Composition of essential oil from seeds of Nigella sativa L. cultivated in Poland. Flav Frag J. 2008; 23:126-132.
• [23]Khan SH, Ansari J, Abbas G. Black cumin seeds as phytogenic product in broiler diets and its effects on performance blood constituents immunity and caecal microbial population. Ital J Anim Sci. 2012; 11:e77.
• [24]Wu T, Qi X, Liu Y, Guo J, Zhu R, Chen W, Yu T. Dietary supplementation with purified mulberry (Morus australis Poir) anthocyanins suppresses body weight gain in high-fat diet fed C57BL/6 mice. Food Chem. 2013; 141:482-487.
• [25]Lönn ME, Dennis JM, Stocker R. Actions of ‘antioxidants’ in the protection against atherosclerosis. Free Radic Biol Med. 2012; 53:863-884.
• [26]Al-Othman AM, Ahmad F, Al-Orf SK, Al-Murshed AZ. Effect of dietary supplementation of Ellataria cardamomum and Nigella sativa on the toxicity of rancid corn oil in Rats. Int J Pharmacol. 2006; 2:60-65.
• [27]Alenzi FQ, El-Bolkiny YS, Salem ML. Protective effects of Nigella sativa oil and thymoquinone against toxicity induced by the anticancer drug cyclophosphamide. Br J Biomed Sci. 2010; 67:20-28.
• [28]Meral I, Kanter M. Effects of Nigella sativa L. and Urtica dioica L. on selected mineral status and hematological values in CCl 4 -treated rats. Biol Trace Elem Res. 2003; 96:263-270.
• [29]El-Desoky G, Abdelreheem M, Abdulaziz AL, ALOthman Z, Mahmoud M, Yusuf K. Potential hepatoprotective effects of vitamin E and selenium on hepatotoxicity induced by malathion in rats. Af J Pharm Pharmacol. 2012; 6:806-813.
• [30]Sánchez-Reus MI, del Rio G, Iglesias MA, Elorza I, Slowing M, Benedí K. Standardized Hypericum perforatum reduces oxidative stress and increases gene expression of antioxidant enzymes on rotenone-exposed rats. Neuropharmacology. 2007; 52:606-616.
• [31]Das L, Bhaumik E, Raychaudhuri U, Chakraborty R. Role of nutraceuticals in human health. J Food Sci Technol. 2012; 49:173-183.
• [32]Casalino E, Sblano C, Calzaretti G, Landriscina C. Acute cadmium intoxication induces alpha-class glutathione S-transferase protein synthesis and enzyme activity in rat liver. Toxic. 2006; 217:240-245.