Antitumor mechanisms of S-allyl mercaptocysteine for breast cancer therapy

BMC Complementary and Alternative Medicine

Research Article

Kaiming Wang¹  Hong Zhang¹  Guimei Lin¹  Zhongxi Zhao¹ 

[1] School of Pharmaceutical Sciences and Center for Pharmaceutical Research & DDS, Shandong University, 250012, Jinan, Shandong, China;

关键词: S-allyl mercaptocysteine;    Breast cancer;    Apoptosis;    Cell cycle arrest;   

DOI  :  10.1186/1472-6882-14-270

 received in 2013-10-31, accepted in 2014-07-22,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundS-allyl mercaptocysteine (SAMC), a water-soluble component derived from garlic, has been found to exert multi-antitumor activities. This study was to investigate the responsible molecular mechanisms of SAMC in human breast cancer cell lines.MethodsSulforhodamine B assay was used to determine cell viability, flow cytometry was applied for the analysis of cell cycle and cell apoptosis, the change of protein was detected by Western blot.ResultsIt was found that SAMC exhibited an effective cell growth inhibition of human breast cancer cell lines MCF-7 (ER positive) and MDA-MB-231 (ER negative) in a dose- and time-dependent manner by inducing cell cycle arrested in G0/G1 phase, the block of cell cycle was associated with the up-regulation of p53 and p21. Furthermore, the SAMC-mediated cell cycle arrest was accompanied with promotion of apoptosis, as indicated by the changes in the nuclear morphology and expressions of apoptosis-related proteins. SAMC clearly triggered the mitochondrial apoptotic pathway as indicated by activation of Bax, decreased expression of Bcl-2 and Bcl-XL, and subsequent activation of caspase-9 and caspase-3.ConclusionThese results highlight the value of a continued investigation into the use of SAMC as a potential antitumor candidate for breast cancer.

【 授权许可】

Unknown   
© Zhang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.