Detection of HPV DNA in esophageal cancer specimens from different regions and ethnic groups: a descriptive study

BMC Cancer

Research Article

Changdong Lu¹  Xiaotian Shi¹  Zhong Wang¹  Min Sun²  Xiuyun Tian²  Xueqian Wang²  Fangfang Liu²  Yiqiang Zhao²  Hong Cai²  Yang Ke³  Curtis C Harris⁴  Qingying Zhang⁵  Zhongying Shen⁵  Donghong Zhang⁵  Dafang Chen⁶  Feng Li⁷ 

[1] Anyang Cancer Hospital, 455000, Anyang, Henan, China;Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University School of Oncology, Beijing Cancer Hospital & Institute, 52 Fucheng Rd, 100142, Beijing, China;Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University School of Oncology, Beijing Cancer Hospital & Institute, 52 Fucheng Rd, 100142, Beijing, China;Department of Cell Biology, Health Science Center, Peking University, 38 Xueyuan Rd, 100191, Beijing, China;Laboratory of Human Carcinogenesis, NCI, NIH, 37 Convent Dr, 20892-4255, Bethesda, MD, USA;Medical School, Shantou University, 22 Xinling Rd, 515031, Shantou, Guangdong, China;School of Public Health, Health Science Center, Peking University, 38 Xueyuan Rd, 100191, Beijing, China;Shihezi Medical School, North Second Rd, 832002, Shihezi, Xinjiang, China;

关键词: Cervical Cancer;    Esophageal Cancer;    Esophageal Squamous Cell Carcinoma;    Esophageal Carcinoma;    Esophageal Carcinogenesis;   

DOI  :  10.1186/1471-2407-10-19

 received in 2009-07-31, accepted in 2010-01-16,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundHPV has been found repeatedly in esophageal carcinoma tissues. However, reported detection rates of HPV DNA in these tumors have varied markedly. Differences in detection methods, sample types, and geographic regions of sample origin have been suggested as potential causes of this discrepancy.MethodsHPV L1 DNA and HPV genotypes were evaluated in 435 esophageal carcinoma specimens collected from four geographic regions with different ethnicities including Anyang in north China, Shantou in south China, Xinjiang in west China, and the United States. The HPV L1 fragment was detected using SPF1/GP6+ primers. HPV genotyping was performed using genotype specific PCR.ResultsTwo hundred and forty four of 435 samples (56.1%) tested positive for HPV L1. Significant differences in detection rate were observed neither among the three areas of China nor between China and the US. HPV6, 16, 18, 26, 45, 56, 57, and 58 were identified in L1 positive samples. HPV16 and 57 were the most common types in all regions, followed by HPV26 and HPV18.ConclusionsHPV infection is common in esophageal carcinoma independent of region and ethnic group of origin. Findings in this study raise the possibility that HPV is involved in esophageal carcinogenesis. Further investigation with a larger sample size over broader geographic areas may be warranted.

【 授权许可】

Unknown   
© Wang et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.