P66shc and its downstream Eps8 and Rac1 proteins are upregulated in esophageal cancers

Cell Communication and Signaling

Short Report

Mohammad A Zargar¹  Deeba Kirmani¹  Rafia A Baba²  Hina F Bhat²  Firdous A Khanday²  Muneesa Bashir²  Khurshid I Andrabi²  Rouf Hamza³  Nisar A Wani⁴  Sameer Naqash⁵ 

[1] Department of Biochemistry, University of Kashmir, Jammu and Kashmir, India;Department of Biotechnology, University of Kashmir, Jammu and Kashmir, India;Department of Botany, University of Kashmir, Jammu and Kashmir, India;Department of Surgery, Government Medical College, SMHS hospital, Srinagar, Jammu and Kashmir, India;Department of Surgery, Sheri-Kashmir Institute of Medical Sciences, Soura, Jammu and Kashmir, India;

关键词: Esophageal Cancer;    Esophageal Squamous Cell Carcinoma;    Esophageal Carcinoma;    Severe Oxidative Stress;    Rac1 Protein;   

DOI  :  10.1186/1478-811X-8-13

 received in 2010-02-17, accepted in 2010-06-18,  发布年份 2010

来源: Springer

【 摘 要 】

Members of Shc (src homology and collagen homology) family, p46shc, p52shc, p66shc have known to be related to cell proliferation and carcinogenesis. Whereas p46shc and p52shc drive the reaction forward, the role of p66shc in cancers remains to be understood clearly. Hence, their expression in cancers needs to be evaluated carefully so that Shc analysis may provide prognostic information in the development of carcinogenesis. In the present study, the expression of p66shc and its associate targets namely Eps8 (epidermal pathway substrate 8), Rac1 (ras-related C3 botulinum toxin substrate1) and Grb2 (growth factor receptor bound protein 2) were examined in fresh tissue specimens from patients with esophageal squamous cell carcinoma and esophageal adenocarcinoma using western blot analysis. A thorough analysis of both esophageal squamous cell carcinoma and adenocarcinoma showed p66shc expression to be significantly higher in both types of carcinomas as compared to the controls. The controls of adenocarcinoma show a higher basal expression level of p66shc as compared to the controls of squamous cell carcinoma. The expression level of downstream targets of p66shc i.e., eps8 and rac1 was also found to be consistently higher in human esophageal carcinomas, and hence correlated positively with p66shc expression. However the expression of grb2 was found to be equal in both esophageal squamous cell carcinoma and adenocarcinoma. The above results suggest that the pathway operated by p66shc in cancers does not involve the participation of Ras and Grb2 as downstream targets instead it operates the pathway involving Eps8 and Rac1 proteins. From the results it is also suggestive that p66shc may have a role in the regulation of esophageal carcinomas and represents a possible mechanism of signaling for the development of squamous cell carcinoma and adenocarcinoma of esophagus.

【 授权许可】

Unknown   
© Bashir et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.