The role of invertases in plant compensatory responses to simulated herbivory

BMC Plant Biology

Research Article

Ken N. Paige¹  Madhura H. Siddappaji¹  Daniel R. Scholes²  Sindhu M. Krishnankutty³  Raymond E. Zielinski⁴  Bernarda Calla⁵  Steven J. Clough⁶ 

[1] Department of Animal Biology, University of Illinois, 61801, Urbana, IL, USA;Department of Animal Biology, University of Illinois, 61801, Urbana, IL, USA;Department of Biology, University of Indianapolis, 46227, Indianapolis, IN, USA;Department of Entomology, University of Illinois, 61801, Urbana, IL, USA;Department of Plant Biology, University of Illinois, 61801, Urbana, IL, USA;USDA-Agricultural Research Service Pacific Basin, Agricultural Research Center, 96720, Hilo, HI, USA;USDA-Agricultural Research Service and the Department of Crop Sciences, University of Illinois, 61801, Urbana, IL, USA;

关键词: Arabidopsis;    Invertase;    Overcompensation;    Herbivory;    G6PD1;    Sucrose;    Oxidative pentose phosphate pathway;   

DOI  :  10.1186/s12870-015-0655-6

 received in 2015-07-09, accepted in 2015-10-23,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe ability of a plant to overcome animal-induced damage is referred to as compensation or tolerance and ranges from undercompensation (decreased fitness when damaged) to overcompensation (increased fitness when damaged). Although it is clear that genetic variation for compensation exists among plants, little is known about the specific genetic underpinnings leading to enhanced fitness. Our previous study identified the enzyme GLUCOSE-6-PHOSPHATE DEHYDROGENASE 1 (G6PD1) as a key regulator contributing to the phenomenon of overcompensation via its role in the oxidative pentose phosphate pathway (OPPP). Apart from G6PD1 we also identified an invertase gene which was up-regulated following damage and that potentially integrates with the OPPP. The invertase family of enzymes hydrolyze sucrose to glucose and fructose, whereby the glucose produced is shunted into the OPPP and presumably supports plant regrowth, development, and ultimately compensation. In the current study, we measured the relative expression of 12 invertase genes over the course of plant development in the Arabidopsis thaliana genotypes Columbia-4 and Landsberg erecta, which typically overcompensate and undercompensate, respectively, when damaged. We also compared the compensatory performances of a set of invertase knockout mutants to the Columbia-4 wild type.ResultsWe report that Columbia-4 significantly up-regulated 9 of 12 invertase genes when damaged relative to when undamaged, and ultimately overcompensated for fruit production. Landsberg erecta, in contrast, down-regulated two invertase genes following damage and suffered reduced fitness. Knockout mutants of two invertase genes both exhibited significant undercompensation for fruit production, exhibiting a complete reversal of the wild type Col-4’s overcompensation.ConclusionCollectively, these results confirm that invertases are essential for not only normal plant growth and development, but also plants’ abilities to regrow and ultimately compensate for fitness following apical damage.

【 授权许可】

CC BY   
© Siddappaji et al. 2015