Phytochemical and pharmacological evaluation of ethanolic extract of Lepisanthes rubiginosa L. leaves

BMC Complementary and Alternative Medicine

Research Article

Md. Mustafizur Rahman¹  Abdullah Shamim¹  Md. Mahedi Hasan¹  Amir Hossain² 

[1] Pharmacy Discipline, Life Science School, Khulna University, 9208, Khulna, Bangladesh;Pharmacy Discipline, Life Science School, Khulna University, 9208, Khulna, Bangladesh;Department of Pharmacy, Dhaka International University, Satarkul, Badda, Dhaka, Bangladesh;

关键词: Lepisanthes rubiginosa;    Antioxidants;    Analgesic;    Antihyperglycemic;    Antidiarrheal;    Neuropharmacological;    Mice;   

DOI  :  10.1186/s12906-017-2010-y

 received in 2017-05-28, accepted in 2017-11-15,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe current study was conducted to evaluate the antioxidant, analgesic, antihyperglycemic, neuropharmacological and antidiarrheal activities of ethanolic extract of Lepisanthes rubiginosa L. leaves in different experimental models.MethodsQuantitative and qualitative analysis were done by TLC (thin layer chromatography) and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging assay. Analgesic, antihyperglycemic and antidiarrheal activities were evaluated using acetic acid induced writhing in mice, oral glucose tolerance test and castor oil induced diarrhea, respectively. Neuropharmacological activity was investigated in mice using both Open Field and Hole Board methods.ResultsTLC analysis indicated the presence of antioxidant compounds in the extract we used. The extract showed IC50 value was 31.62 μg/mL whereas the standard ascorbic acid showed 12.02 μg/mL. In acetic acid induced writhing assay, the extract showed 46.07% and 58.43% writhing inhibition at the doses of 250 mg/kg and 500 mg/kg body weight, respectively whereas standard diclofenac-Na (25 mg/kg) showed 86.52% writhing inhibition. The plant extract showed significant (p < 0.05) antihyperglycemic activity on mice as compared to control groups. In neuropharmacological activity assay the experimental animal showed a noticeable decrease in locomotion by showing a decrease in number of square crossed and head dipping at both doses (250 mg/kg & 500 mg/kg). In antidiarrheal activity test, the plant extract at the doses of 250 mg/kg and 500 mg/kg showed percent inhibition of defecation 57.89 and 77.19 respectively, whereas standard loperamide (3 mg/kg) showed percent inhibition of defecation 88.59.ConclusionThe results demonstrated that the extract has potential antioxidant, analgesic, antihyperglycemic, neuropharmacological and antidiarrheal activity.

【 授权许可】

CC BY   
© The Author(s). 2017