RNAi-mediated down-regulation of the expression of OsFAD2-1: effect on lipid accumulation and expression of lipid biosynthetic genes in the rice grain

BMC Plant Biology

Research Article

Qing Liu¹  Zhongyi Li¹  Pushkar Shreshtha¹  Sadequr Rahman²  Gopal Ji Tiwari² 

[1] CSIRO Agriculture & Food, PO Box 1600, 2601, Canberra, ACT, Australia;School of Science, Monash University Malaysia, 46150 Bandar Sunway, Selangor, Malaysia;Monash University Malaysia Genomics Facility, 46150 Bandar Sunway, Selangor, Malaysia;

关键词: Rice bran oil;    Triacylglycerol;    Oleic acid;    FAD2;    Transcriptome;   

DOI  :  10.1186/s12870-016-0881-6

 received in 2016-07-09, accepted in 2016-08-24,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe bran from polished rice grains can be used to produce rice bran oil (RBO). High oleic (HO) RBO has been generated previously through RNAi down-regulation of OsFAD2-1. HO-RBO has higher oxidative stability and could be directly used in the food industry without hydrogenation, and is hence free of trans fatty acids. However, relative to a classic oilseed, lipid metabolism in the rice grain is poorly studied and the genetic alteration in the novel HO genotype remains unexplored.ResultsHere, we have undertaken further analysis of role of OsFAD2-1 in the developing rice grain. The use of Illumina-based NGS transcriptomics analysis of developing rice grain reveals that knockdown of Os-FAD2-1 gene expression was accompanied by the down regulation of the expression of a number of key genes in the lipid biosynthesis pathway in the HO rice line. A slightly higher level of oil accumulation was also observed in the HO-RBO.ConclusionProminent among the down regulated genes were those that coded for FatA, LACS, SAD2, SAD5, caleosin and steroleosin. It may be possible to further increase the oleic acid content in rice oil by altering the expression of the lipid biosynthetic genes that are affected in the HO line.

【 授权许可】

CC BY   
© The Author(s). 2016