期刊论文详细信息
BMC Microbiology
Transposon insertion sequencing reveals T4SS as the major genetic trait for conjugation transfer of multi-drug resistance pEIB202 from Edwardsiella
Research Article
Yang Liu1  Yanan Gao1  Qiyao Wang2  Xiaohong Liu2  Yuanxing Zhang2  Jingfan Xiao2  Qin Liu2 
[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China;Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, China;Shanghai Collaborative Innovation Center for Biomanufacturing, 130 Meilong Road, 200237, Shanghai, China;
关键词: Plasmid conjugation;    Edwardsiella Piscicida;    Tis;    T4SS;    RNA-seq;    EsrB;   
DOI  :  10.1186/s12866-017-1013-7
 received in 2017-01-18, accepted in 2017-04-26,  发布年份 2017
来源: Springer
PDF
【 摘 要 】

BackgroundConjugation is a major type of horizontal transmission of genes that involves transfer of a plasmid into a recipient using specific conjugation machinery, which results in an extended spectrum of bacterial antibiotics resistance. However, there is inadequate knowledge about the regulator and mechanisms that control the conjugation processes, especially in an aquaculture environment where a cocktail of antibiotics may be present. Here, we investigated these with pEIB202, a typical multi-drug resistant IncP plasmid encoding tetracycline, streptomycin, sulfonamide and chloramphenicol resistance in fish pathogen Edwardsiella piscicida strain EIB202.ResultsWe used transposon insertion sequencing (TIS) to identify genes that are responsible for conjugation transfer of pEIB202. All ten of the plasmid-borne type IV secretion system (T4SS) genes and a putative lipoprotein p007 were identified to play an important role in pEIB202 horizontal transfer. Antibiotics appear to modulate conjugation frequencies by repressing T4SS gene expression. In addition, we identified topA gene, which encodes topoisomerase I, as an inhibitor of pEIB202 transfer. Furthermore, the RNA-seq analysis of the response regulator EsrB encoded on the chromosome also revealed its essential role in facilitating the conjugation by upregulating the T4SS genes.ConclusionsCollectively, our screens unraveled the genetic basis of the conjugation transfer of pEIB202 and the influence of horizontally acquired EsrB on this process. Our results will improve the understanding of the mechanism of plasmid conjugation processes that facilitate dissemination of antibiotic resistance especially in aquaculture industries.

【 授权许可】

CC BY   
© The Author(s). 2017

【 预 览 】
附件列表
Files Size Format View
RO202311094927393ZK.pdf 1503KB PDF download
【 参考文献 】
  • [1]
  • [2]
  • [3]
  • [4]
  • [5]
  • [6]
  • [7]
  • [8]
  • [9]
  • [10]
  • [11]
  • [12]
  • [13]
  • [14]
  • [15]
  • [16]
  • [17]
  • [18]
  • [19]
  • [20]
  • [21]
  • [22]
  • [23]
  • [24]
  • [25]
  • [26]
  • [27]
  • [28]
  • [29]
  • [30]
  • [31]
  • [32]
  • [33]
  • [34]
  • [35]
  • [36]
  • [37]
  • [38]
  • [39]
  • [40]
  • [41]
  • [42]
  • [43]
  • [44]
  • [45]
  • [46]
  • [47]
  • [48]
  • [49]
  • [50]
  • [51]
  • [52]
  • [53]
  • [54]
  • [55]
  文献评价指标  
  下载次数:7次 浏览次数:1次