Complete-genome sequencing and comparative genomic characterization of blaNDM-5 carrying Citrobacter freundii isolates from a patient with multiple infections

BMC Genomics

Research

Lulu Jin¹  Maofeng Wang¹  Jianzhong Ye²  Tieli Zhou²  Zhongyong Wang²  Yishuai Lin²  Yaling Li³  Beiwen Zheng⁴  Hao Xu⁵ 

[1] Department of Biomedical Sciences Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, No. 60 Wuning West Road, 322100, Dongyang, Zhejiang Province, China;Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, the First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang Street, Ouhai District, 325000, Wenzhou, Zhejiang Province, China;Department of Health Screening Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, 310000, Hangzhou, Zhejiang, China;State Key Laboratory for Diagnosis and Treatment of Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesSchool of Medicine, National Clinical Research Center for Infectious Diseasesthe First Affiliated HospitalZhejiang University, No. 79 Qingchun Road, Shangcheng District, 310000, Hangzhou, Zhejiang Province, China;Wenzhou Medical University, 325000, Wenzhou, Zhejiang, China;State Key Laboratory for Diagnosis and Treatment of Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesSchool of Medicine, National Clinical Research Center for Infectious Diseasesthe First Affiliated HospitalZhejiang University, No. 79 Qingchun Road, Shangcheng District, 310000, Hangzhou, Zhejiang Province, China;

关键词: Antimicrobial resistance;    Horizontal gene transfer;    IncX plasmid;    Plasmid conjugation;   

DOI  :  10.1186/s12864-023-09579-9

 received in 2023-01-26, accepted in 2023-08-11,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe emergence and wide spread of carbapenemase-producing Enterobacteriaceae (CPE) poses a growing threat to global public health. However, clinically derived carbapenemase-producing Citrobacter causing multiple infections has rarely been investigated. Here we first report the isolation and comparative genomics of two blaNDM-5 carrying Citrobacter freundii (C. freundii) isolates from a patient with bloodstream and urinary tract infections.ResultsAntimicrobial susceptibility testing showed that both blaNDM-5 carrying C. freundii isolates were multidrug-resistant. Positive modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) results suggested metallo-carbapenemase production. PCR and sequencing confirmed that both metallo-carbapenemase producers were blaNDM-5 positive. Genotyping and comparative genomics analyses revealed that both isolates exhibited a high level of genetic similarity. Plasmid analysis confirmed that the blaNDM-5 resistance gene is located on IncX3 plasmid with a length of 46,161 bp, and could successfully be transferred to the recipient Escherichia coli EC600 strain. A conserved structure sequence (ISAba125-IS5-blaNDM-5-trpF-IS26-umuD-ISKox3) was found in the upstream and downstream of the blaNDM-5 gene.ConclusionsThe data presented in this study showed that the conjugative blaNDM-5 plasmid possesses a certain ability to horizontal transfer. The dissemination of NDM-5-producing C. freundii isolates should be of close concern in future clinical surveillance. To our knowledge, this is the first study to characterize C. freundii strains carrying the blaNDM-5 gene from one single patient with multiple infections.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

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