Genotyping and drug susceptibility testing of mycobacterial isolates from population-based tuberculosis prevalence survey in Ghana

BMC Infectious Diseases

Research Article

Kennedy Kwasi Addo¹  Gloria Ivy Mensah¹  Samuel Ofori Addo²  Frank Adae Bonsu³  Lydia Mosi⁴ 

[1] Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, P.O. Box LG 581, Legon, Ghana;Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, P.O. Box LG 581, Legon, Ghana;West African Center for Cell Biology of Infectious Pathogens, University of Ghana, Legon, Ghana;National Tuberculosis Programme, Ghana Health Service, Accra, Ghana;West African Center for Cell Biology of Infectious Pathogens, University of Ghana, Legon, Ghana;Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon, Ghana;

关键词: Tuberculosis;    Prevalence survey;    Ghana;    Line probe assay;    Drug resistance;    plus;    NTM-DR;   

DOI  :  10.1186/s12879-017-2853-3

 received in 2017-02-04, accepted in 2017-11-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMycobacterium tuberculosis complex (MTBC) and Non-tuberculosis Mycobacterium (NTM) infections differ clinically, making rapid identification and drug susceptibility testing (DST) very critical for infection control and drug therapy. This study aims to use World Health Organization (WHO) approved line probe assay (LPA) to differentiate mycobacterial isolates obtained from tuberculosis (TB) prevalence survey in Ghana and to determine their drug resistance patterns.MethodsA retrospective study was conducted whereby a total of 361 mycobacterial isolates were differentiated and their drug resistance patterns determined using GenoType Mycobacterium Assays: MTBC and CM/AS for differentiating MTBC and NTM as well MTBDRplus and NTM-DR for DST of MTBC and NTM respectively.ResultsOut of 361 isolates, 165 (45.7%) MTBC and 120 (33.2%) NTM (made up of 14 different species) were identified to the species levels whiles 76 (21.1%) could not be completely identified. The MTBC comprised 161 (97.6%) Mycobacterium tuberculosis and 4 (2.4%) Mycobacterium africanum. Isoniazid and rifampicin monoresistant MTBC isolates were 18/165 (10.9%) and 2/165(1.2%) respectively whiles 11/165 (6.7%) were resistant to both drugs. Majority 42/120 (35%) of NTM were M. fortuitum. DST of 28 M. avium complex and 8 M. abscessus complex species revealed that all were susceptible to macrolides (clarithromycin, azithromycin) and aminoglycosides (kanamycin, amikacin, and gentamicin).ConclusionOur research signifies an important contribution to TB control in terms of knowledge of the types of mycobacterium species circulating and their drug resistance patterns in Ghana.

【 授权许可】

CC BY   
© The Author(s). 2017