Effect on the tensile strength of human acellular dermis (Epiflex®) of in-vitro incubation simulating an open abdomen setting

BMC Surgery

Research Article

Matthias Oechsner¹  Michael Mularczyk¹  Hans Haupt¹  Eric Dominic Rössner²  Peter Hohenberger²  Florian Herrle²  Mario Vitacolonna²  Torsten J Schulze³  Lothar R Pilz⁴ 

[1] Center for structural Materials, State Material Testing Institute Darmstadt (MPA), Chair and Institute for Material Science (IfW), Technische Universität Darmstadt, Darmstadt, Germany;Division of Surgical Oncology and Thoracic Surgery, Department of Surgery, University Medical Centre Mannheim, Heidelberg University, Theodor Kutzer Ufer 1-3, 68167, Mannheim, Germany;German Red Cross Blood Service, Baden Württemberg-Hessen, Medical Faculty of Mannheim, Institute of Transfusion Medicine and Immunology, Heidelberg University, Mannheim, Germany;Medical Faculty Mannheim, Heidelberg University, Heidelberg, Germany;

关键词: Acellular dermis;    Open abdomen;    Breaking strength;    Biologicals;   

DOI  :  10.1186/1471-2482-14-7

 received in 2013-06-09, accepted in 2014-01-21,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe use of human acellular dermis (hAD) to close open abdomen in the treatment process of severe peritonitis might be an alternative to standard care. This paper describes an investigation of the effects of fluids simulating an open abdomen environment on the biomechanical properties of Epiflex® a cell-free human dermis transplant.MethodshAD was incubated in Ringers solution, blood, urine, upper gastrointestinal (upper GI) secretion and a peritonitis-like bacterial solution in-vitro for 3 weeks. At day 0, 7, 14 and 21 breaking strength was measured, tensile strength was calculated and standard fluorescence microscopy was performed.ResultshAD incubated in all five of the five fluids showed a decrease in mean breaking strength at day 21 when compared to day 0. However, upper GI secretion was the only incubation fluid that significantly reduced the mechanical strength of Epiflex after 21days of incubation when compared to incubation in Ringer’s solution.ConclusionhAD may be a suitable material for closure of the open abdomen in the absence of upper GI leakage and pancreatic fistulae.

【 授权许可】

Unknown   
© Vitacolonna et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.