The utility of the hematoxylin and eosin staining in patients with suspected Hirschsprung disease

BMC Surgery

Research Article

Didik Setyo Heriyanto¹  Kristy Iskandar²  Josephine Amanda Setiadi³  Andi Dwihantoro³ 

[1] Department of Anatomical Pathology, Faculty of Medicine, Universitas Gadjah Mada/Dr. Sardjito Hospital, 55281, Yogyakarta, Indonesia;Department of Child Health, Universitas Gadjah Mada Hospital, 55291, Yogyakarta, Indonesia;Pediatric Surgery Division, Department of Surgery, Faculty of Medicine, Universitas Gadjah Mada/Dr. Sardjito Hospital, 55281, Yogyakarta, Indonesia;

关键词: Diagnosis;    Hematoxylin and eosin;    Hirschsprung disease;    Histopathology;    Indonesia;    S100;   

DOI  :  10.1186/s12893-017-0267-1

 received in 2017-02-21, accepted in 2017-06-14,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundWhile immunohistochemistry (IHC) methods have been widely conducted for the diagnosis of Hirschsprung disease (HSCR) in developed countries, there are very few studies on their use in developing countries where hematoxylin and eosin (HE) staining is a key element of the diagnosis of HSCR. We aimed to determine the accuracy of HE staining in the diagnosis of HSCR using S100 IHC as the reference standard in Indonesia.MethodsAll histopathology performed for the suspicion of HSCR patients from January 2013 to August 2015 in Dr. Sardjito Hospital, Yogyakarta, Indonesia, were retrospectively reviewed.ResultsOur study included 23 HSCR patients: 9 males and 14 females. The HE staining revealed 14 negative (absence of ganglion cells) cases (61%) and 9 positive (presence of ganglion cells) cases (39%). In S100 IHC, out of the 9 positive cases by HE staining, 6 (67%) were confirmed for having ganglion cells; and out of the 14 negative cases by HE staining, 12 (86%) were reported negative and 2 (14%) were positive by S100 IHC staining. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy rates of the HE staining were 80% (95% CI: 0.51–0.95), 75% (95% CI: 0.36–0.96), 85.7% (95% CI: 0.56–0.98), 66.7% (95% CI: 0.31–0.91), and 78.3% (95% CI: 0.58–0.90), respectively.ConclusionsOur study showed that HE staining has relatively moderate accuracy for the diagnosis of HSCR. The use of HE staining is still recommended for the diagnosis of HSCR given the limitation of resource allocation for more expensive IHC technologies in developing countries.

【 授权许可】

CC BY   
© The Author(s). 2017