Gli2a protein localization reveals a role for Iguana/DZIP1 in primary ciliogenesis and a dependence of Hedgehog signal transduction on primary cilia in the zebrafish

BMC Biology

Research Article

Freek van Eeden¹  Hyejeong Rosemary Kim¹  Philip W Ingham²  Joanna Richardson³ 

[1] MRC Centre for Developmental & Biomedical Genetics, University of Sheffield, S10 2TN, Sheffield, UK;MRC Centre for Developmental & Biomedical Genetics, University of Sheffield, S10 2TN, Sheffield, UK;Institute of Molecular and Cell Biology, Proteos, 61 Biopolis Drive, 138673, Singapore;MRC Centre for Developmental & Biomedical Genetics, University of Sheffield, S10 2TN, Sheffield, UK;MRC Centre for Developmental Neurobiology, New Hunt's House, Guy's Campus, SE1 1UL, London, UK;

关键词: Basal Body;    Zebrafish Embryo;    Primary Cilium;    Mutant Embryo;    Wild Type Embryo;   

DOI  :  10.1186/1741-7007-8-65

 received in 2009-10-09, accepted in 2010-04-19,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundIn mammalian cells, the integrity of the primary cilium is critical for proper regulation of the Hedgehog (Hh) signal transduction pathway. Whether or not this dependence on the primary cilium is a universal feature of vertebrate Hedgehog signalling has remained contentious due, in part, to the apparent divergence of the intracellular transduction pathway between mammals and teleost fish.ResultsHere, using a functional Gli2-GFP fusion protein, we show that, as in mammals, the Gli2 transcription factor localizes to the primary cilia of cells in the zebrafish embryo and that this localization is modulated by the activity of the Hh pathway. Moreover, we show that the Igu/DZIP1protein, previously implicated in the modulation of Gli activity in zebrafish, also localizes to the primary cilium and is required for its proper formation.ConclusionOur findings demonstrate a conserved role of the primary cilium in mediating Hedgehog signalling activity across the vertebrate phylum and validate the use of the zebrafish as a representative model for the in vivo analysis of vertebrate Hedgehog signalling.

【 授权许可】

Unknown   
© Kim et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.