Dual transcriptome of the immediate neutrophil and Candida albicans interplay

BMC Genomics

Research

Madhu Shankar¹  Constantin F. Urban¹  José Pedro Lopes¹  David Ermert²  Maria J. Niemiec³  Christian Grumaz⁴  Kai Sohn⁴  Christiane Desel⁵  Ian G. Mills⁶  Philip Stevens⁷ 

[1] Department of Clinical Microbiology, Umeå Centre for Microbial Research (UCMR) & Laboratory of Molecular Infection Medicine Sweden (MIMS), Umeå University, Umea, Sweden;Department of Clinical Microbiology, Umeå Centre for Microbial Research (UCMR) & Laboratory of Molecular Infection Medicine Sweden (MIMS), Umeå University, Umea, Sweden;Present Address: Division of Medical Protein Chemistry, Department of Translational Medicine, Lund University, Malmö, Sweden;Department of Clinical Microbiology, Umeå Centre for Microbial Research (UCMR) & Laboratory of Molecular Infection Medicine Sweden (MIMS), Umeå University, Umea, Sweden;Present Address: Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Jena, Germany & Center for Sepsis Control and Care (CSCC), Jena, Germany;Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany;Institute of Clinical Microbiology, Immunology and Hygiene, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany;Present Address: The Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK;Prostate Cancer Research Group, Center of Molecular Medicine Norway (NCMM), Oslo, Norway;Department of Molecular Oncology, Institute of Cancer Research, Radium Hospital, Oslo, Norway;PCUK/Movember Centre of Excellence for Prostate Cancer Research, Centre for Cancer Research and Cell Biology (CCRCB), Queen’s University Belfast, Belfast, UK;University of Stuttgart IGVP, Stuttgart, Germany;Center for Integrative Bioinformatics Vienna, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria;

关键词: Candida;    Neutrophils;    Dual transcriptome;    Extracellular traps;    NOD-like receptor pathway;    Nutritional immunity;    Morphotype;   

DOI  :  10.1186/s12864-017-4097-4

 received in 2017-02-13, accepted in 2017-08-30,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundNeutrophils are traditionally considered transcriptionally inactive. Compared to other immune cells, little is known about their transcriptional profile during interaction with pathogens.MethodsWe analyzed the meta-transcriptome of the neutrophil-Candida albicans interplay and the transcriptome of C. albicans challenged with neutrophil extracellular traps (NETs) by RNA-Seq, considering yeast and hypha individually in each approach.ResultsThe neutrophil response to C. albicans yeast and hyphae was dominated by a morphotype-independent core response. However, 11 % of all differentially expressed genes were regulated in a specific manner when neutrophils encountered the hyphal form of C. albicans. While involving genes for transcriptional regulators, receptors, and cytokines, the neutrophil core response lacked typical antimicrobial effectors genes. Genes of the NOD-like receptor pathway, including NLRP3, were enriched.Neutrophil- and NET-provoked responses in C. albicans differed. At the same time, the Candida transcriptome upon neutrophil encounter and upon NET challenge included genes from various metabolic processes and indicate a mutual role of the regulators Tup1p, Efg1p, Hap43p, and Cap1p. Upon challenge with neutrophils and NETs, the overall Candida response was partially morphotype-specific. Yet again, actual oppositional regulation in yeasts and hyphae was only detected for the arginine metabolism in neutrophil-infecting C. albicans.Conclusions Taken together, our study provides a comprehensive and quantitative transcript profile of the neutrophil–C. albicans interaction. By considering the two major appearances of both, neutrophils and C. albicans, our study reveals yet undescribed insights into this medically relevant encounter. Hence, our findings will facilitate future research and potentially inspire novel therapy developments.

【 授权许可】

CC BY   
© The Author(s). 2017