Transcriptome changes during fruit development and ripening of sweet orange (Citrus sinensis)

BMC Genomics

Research Article

Keqin Yu¹  Fei Guo¹  Xinlei Da¹  Yuduan Ding¹  Xiuxin Deng²  Qiang Xu² 

[1] Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, 430070, Wuhan, China;Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, 430070, Wuhan, China;National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070, Wuhan, China;

关键词: Carotenoid;    Lycopene;    Fruit Development;    Citrus Fruit;    Sweet Orange;   

DOI  :  10.1186/1471-2164-13-10

 received in 2011-09-24, accepted in 2012-01-10,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe transcriptome of the fruit pulp of the sweet orange variety Anliu (WT) and that of its red fleshed mutant Hong Anliu (MT) were compared to understand the dynamics and differential expression of genes expressed during fruit development and ripening.ResultsThe transcriptomes of WT and MT were sampled at four developmental stages using an Illumina sequencing platform. A total of 19,440 and 18,829 genes were detected in MT and WT, respectively. Hierarchical clustering analysis revealed 24 expression patterns for the set of all genes detected, of which 20 were in common between MT and WT. Over 89% of the genes showed differential expression during fruit development and ripening in the WT. Functional categorization of the differentially expressed genes revealed that cell wall biosynthesis, carbohydrate and citric acid metabolism, carotenoid metabolism, and the response to stress were the most differentially regulated processes occurring during fruit development and ripening.ConclusionA description of the transcriptomic changes occurring during fruit development and ripening was obtained in sweet orange, along with a dynamic view of the gene expression differences between the wild type and a red fleshed mutant.

【 授权许可】

Unknown   
© Yu et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.