Single nucleotide polymorphisms in the CD40 gene associate with the disease susceptibility and severity in knee osteoarthritis in the Chinese Han population: a case-control study

BMC Musculoskeletal Disorders

Research Article

Ping Wu¹  Fang-Jie Zhang¹  Ming-Hua Sun²  Wen-Feng Xiao³  Wei Luo³  Zhen-Han Deng³  Jian Tian³  Yu-Sheng Li³ 

[1] Department of Emergency Medicine, Xiangya Hospital, Central South University, 410008, Changsha, China;Department of Orthopaedics, Affiliated Hospital, Logistics University of Chinese People’s Armed Police Forces, 300162, Tianjin, China;Department of Orthopaedics, Xiangya Hospital, Central South University, 410008, Changsha, China;

关键词: Knee osteoarthritis;    CD40;    Single nucleotide polymorphism;    Susceptibility;    Severity;    Rs4810485 G > T;    Rs1883832 C > T;   

DOI  :  10.1186/s12891-017-1466-8

 received in 2016-07-14, accepted in 2017-01-18,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThis study explored the association between single nucleotide polymorphisms (SNPs) in the CD40 gene, rs4810485 G > T and rs1883832 C > T, as well as disease susceptibility and severity in knee osteoarthritis (KOA) in the Chinese Han population.MethodPeripheral venous blood was collected from 133 KOA patients (KOA group) and 143 healthy people (control group) from December 2012 to November 2013. The patients in the KOA group were classified into mild, moderate and severe groups according to disease severity. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) was used to test the genotypes of all subjects. Binary logistic regression analyses were performed to analyze the risk factors for KOA.ResultsThe KOA group was significantly different from the control group in living environment (P < 0.05). The KOA group had a lower frequency of TT genotype and T allele distribution of rs4810485 G > T compared with the control group, and rs4810485 G > T TT genotype and T allele may associate with low incidence of KOA (all P < 0.05). Besides, T allele and mutant homozygous TT genotype of rs1883832 C > T increased the susceptibility to KOA. Genotype and allele distribution of rs4810485 G > T and rs1883832 C > T were significantly different among the mild, moderate and severe groups (P < 0.05). There were more patients with rs4810485 G > T GG genotype and rs1883832 C > T TT genotype in the severe group than other genotypes of these two SNPs. According to binary logistic regression analysis, rs4810485 G > T TT genotype could alleviate disease severity in KOA, rs1883832 C > T TT genotype increase the severity of KOA and living environment is an important external factor that affects KOA severity.ConclusionsThese data provide evidences that rs4810485 G > T and rs1883832 C > T in the CD40 gene may be associated with disease susceptibility and severity in KOA.

【 授权许可】

CC BY   
© The Author(s). 2017