Genome-wide identification and analysis of mitogen activated protein kinase kinase kinase gene family in grapevine (Vitis vinifera)

BMC Plant Biology

Research Article

Min Wang¹  Ying-Hai Liang¹  Yuan-Chun Ma¹  Zong-Ming Cheng²  Gang Wang³  Annalisa Polverari⁴  Arianna Lovato⁴ 

[1] College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, China;College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, China;Department of Plant Sciences, University of Tennessee, 37996, Knoxville, TN, USA;College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, China;Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, 210014, Nanjing, Jiangsu, China;Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy;

关键词: Grapevine;    Mitogen-activated protein kinase kinase kinase (MAPKKK);    Gene family;    Phylogenetic analysis;    Expression analysis;    Stresses;   

DOI  :  10.1186/s12870-014-0219-1

 received in 2014-04-29, accepted in 2014-08-04,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundMitogen-activated protein kinase kinase kinases (MAPKKKs; MAP3Ks) are important components of MAPK cascades, which are highly conserved signal transduction pathways in animals, yeast and plants, play important roles in plant growth and development. MAPKKKs have been investigated on their evolution and expression patterns in limited plants including Arabidopsis, rice and maize.ResultsIn this study, we performed a genome-wide survey and identified 45 MAPKKK genes in the grapevine genome. Chromosome location, phylogeny, gene structure and conserved protein motifs of MAPKKK family in grapevine have been analyzed to support the prediction of these genes. In the phylogenetic analysis, MAPKKK genes of grapevine have been classified into three subgroups as described for Arabidopsis, named MEKK, ZIK and RAF, also confirmed in grapevine by the analysis of conserved motifs and exon-intron organizations. By analyzing expression profiles of MAPKKK genes in grapevine microarray databases, we highlighted the modulation of different MAPKKKs in different organs and distinct developmental stages. Furthermore, we experimentally investigated the expression profiles of 45 grape MAPKKK genes in response to biotic (powdery mildew) and abiotic stress (drought), as well as to hormone (salicylic acid, ethylene) and hydrogen peroxide treatments, and identified several candidate MAPKKK genes that might play an important role in biotic and abiotic responses in grapevine, for further functional characterization.ConclusionsThis is the first comprehensive experimental survey of the grapevine MAPKKK gene family, which provides insights into their potential roles in regulating responses to biotic and abiotic stresses, and the evolutionary expansion of MAPKKKs is associated with the diverse requirement in transducing external and internal signals into intracellular actions in MAPK cascade in grapevine.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.