Anti-inflammatory effects of Polygonum minus (Huds) extract (Lineminus™) in in-vitro enzyme assays and carrageenan induced paw edema

BMC Complementary and Alternative Medicine

Research Article

Annie George¹  Sasikala Chinnappan¹  Meena Chintamaneni²  Vandana Kotak C²  Yogendra Choudhary²  Ammu K Radhakrishnan³  Thomas Kueper⁴ 

[1] Biotropics Malaysia Berhad, Lot 21 Jalan U1/19, Section U1, Hicom-Glenmarie Industrial Park, 40150, Shah Alam, Selangor, Malaysia;Ethix Pharma, Division of Toxicology and Clinical Affairs, Karbala Road, 495001, Bilaspur, Chhattisgarh, India;Pathology Division, Faculty of Medicine and Health, International Medical University, Kuala Lumpur, Malaysia;Stephanstrasse 28, 48734, Reken, Germany;

关键词: Inflammation;    Polygonum minus;    Paw edema;    Cyclooxygenase;    Lipooxygenase;    Secretory phospholipase-A2;   

DOI  :  10.1186/1472-6882-14-355

 received in 2014-02-12, accepted in 2014-09-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe study was aimed to evaluate the anti-inflammatory activity of ethanolic and aqueous extracts of Polygonum minus (Huds) using in vitro and in vivo approaches.MethodsThe in vitro tests used to evaluate ethanolic extract are cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), lipooxygenase (5-LOX), secretory phospholipase-A2 (sPLA2) inhibition assay whilst the in-vivo effect was measured by the ability of aqueous extracts to reduce paw edema induced by λ-carrageenan, in rats.ResultsThe ethanolic extract inhibited the activities of 5-LOX and COX-1(p < 0.05) whilst the inhibitory effect on COX-2 was only moderate. A marked inhibition of 5-LOX was observed at 30 μg/ ml. The extract did not inhibit the activity of sPLA2. The ability of the ethanolic extracts of Polygonum minus to inhibit both 5-LOX and COX, prompted a study to evaluate the effects of using an aqueous extract of Polygonum minus( LineminusTM); as this would be more suitable for future clinical testing. The anti-inhibitory activity of the aqueous extract from this plant was evaluated using a rat model where inflammation was induced in the paws by injection of λ-carrageenan. The aqueous extracts from Polygonum minus administered at doses of 100 and 300 mg/kg body weight (b.w.), significantly (p < 0.01) reduced paw edema induced by λ-carrageenan in the experimental model, at 4 h compared to the vehicle control. Furthermore, administration of 100 mg/kg b.w. or 300 mg/kg b.w. completely reduced inflammation of the paw 4 h after injection.ConclusionThese findings suggest that aqueous extract of Polygonum minus possesses potent anti-inflammatory activities.

【 授权许可】

CC BY   
© George et al.; licensee BioMed Central Ltd. 2014