Anti-inflammatory effects of Polygonum minus (Huds) extract (Lineminus™) in in-vitro enzyme assays and carrageenan induced paw edema

BMC Complementary and Alternative Medicine

Ammu K Radhakrishnan⁴  Thomas Kueper¹  Yogendra Choudhary²  Vandana Kotak C²  Meena Chintamaneni²  Sasikala Chinnappan³  Annie George³ 

[1] Stephanstrasse 28, 48734 Reken, Germany;Ethix Pharma, Division of Toxicology and Clinical Affairs, Karbala Road, Bilaspur 495001, Chhattisgarh, India;Biotropics Malaysia Berhad, Lot 21 Jalan U1/19, Section U1, Hicom-Glenmarie Industrial Park, 40150 Shah Alam, Selangor, Malaysia;Pathology Division, Faculty of Medicine and Health, International Medical University, Kuala Lumpur, Malaysia

关键词: Secretory phospholipase-A2;    Lipooxygenase;    Cyclooxygenase;    Paw edema;    Polygonum minus (Huds);    Inflammation;   

Others  :  1086563
DOI  :  10.1186/1472-6882-14-355

 received in 2014-02-12, accepted in 2014-09-19,  发布年份 2014

【 摘 要 】

Background

The study was aimed to evaluate the anti-inflammatory activity of ethanolic and aqueous extracts of Polygonum minus (Huds) using in vitro and in vivo approaches.

Methods

The in vitro tests used to evaluate ethanolic extract are cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), lipooxygenase (5-LOX), secretory phospholipase-A2 (sPLA2) inhibition assay whilst the in-vivo effect was measured by the ability of aqueous extracts to reduce paw edema induced by λ-carrageenan, in rats.

Results

The ethanolic extract inhibited the activities of 5-LOX and COX-1(p < 0.05) whilst the inhibitory effect on COX-2 was only moderate. A marked inhibition of 5-LOX was observed at 30 μg/ ml. The extract did not inhibit the activity of sPLA2. The ability of the ethanolic extracts of Polygonum minus to inhibit both 5-LOX and COX, prompted a study to evaluate the effects of using an aqueous extract of Polygonum minus(Lineminus^TM); as this would be more suitable for future clinical testing. The anti-inhibitory activity of the aqueous extract from this plant was evaluated using a rat model where inflammation was induced in the paws by injection of λ-carrageenan. The aqueous extracts from Polygonum minus administered at doses of 100 and 300 mg/kg body weight (b.w.), significantly (p < 0.01) reduced paw edema induced by λ-carrageenan in the experimental model, at 4 h compared to the vehicle control. Furthermore, administration of 100 mg/kg b.w. or 300 mg/kg b.w. completely reduced inflammation of the paw 4 h after injection.

Conclusion

These findings suggest that aqueous extract of Polygonum minus possesses potent anti-inflammatory activities.

【 授权许可】

   
2014 George et al.; licensee BioMed Central Ltd.

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