Evaluation of anti-inflammatory activity of compounds isolated from the rhizome of Ophiopogon japonicas

BMC Complementary and Alternative Medicine

Research Article

Qi Wang¹  Li Lin¹  Chang-Sheng Deng¹  Ding-Sheng Chen²  Jing-Wen Zhao³  Wei Zhu³ 

[1] Artepharm Company Limited, 510410, Guangzhou, Guangdong, China;Guangdong Food and Drug Vocational College, 510520, Guangzhou, Guangdong, China;The Second Institute of Clinical Medicine, Guangzhou University of Chinese Medicine, 510405, Guangzhou, Guangdong, China;

关键词: Ophiopogon japonicus;    Homoisoflavonoids;    Anti-inflammatory;    Macrophages;    MAPKs;   

DOI  :  10.1186/s12906-016-1539-5

 received in 2016-04-27, accepted in 2016-12-12,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundOphiopogon japonicas (L.f) Ker-Gawl has been used as a traditional Chinese medicine to cure acute and chronic inflammation and cardiovascular diseases including thrombotic diseases for thousands of years. Previous phytochemical studies showed that O. japonicus contained compounds with anti-inflammatory activity. The aim of this study was to identify and isolate compounds with anti-inflammatory activity from the rhizome of O. japonicas.MethodsCompounds were isolated by various column chromatography and their structures were identified in terms of nuclear magnetic resonance spectrum (NMR) and mass spectrum (MS). To measure the anti-inflammatory effects of thirteen compounds in LPS-induced RAW 264.7 macrophage cells, we used the following methods: cell viability assay, nitric oxide assay, enzyme-linked immunosorbent assay, quantitative real-time PCR analysis and western blotting analysis.ResultsOne new and twelve known compounds (mainly homoisoflavonoids) were extracted from O. japonicas, in which 4′-O-Demethylophiopogonanone E (10) was considered as a new compound, additionally, compounds 4-O-(2-Hydroxy-1- hydroxymethylethyl)-dihydroconiferyl alcohol (2) and 5,7-dihydroxy-6-methyl-3-(2′, 4′-dihydroxybenzyl) chroman-4-one (12) were isolated from the rhizome of O. japonicas for the first time. The isolated compounds Oleic acid (3), Palmitic acid (4), desmethylisoophiopogonone B [5,7-dihydroxy-3-(4′-hydroxybenzyl)-8- methyl- chromone] (5), 5,7-dihydroxy-6-methyl-3-(4′-hydroxybenzyl) chromone (7) and 10 significantly suppressed the production of NO in LPS-induced RAW 264.7 cells. Especially compound 10 showed the strongest effect against the production of the pro-inflammatory cytokine IL-1β and IL-6 with the IC50 value of 32.5 ± 3.5 μg/mL and 13.4 ± 2.3 μg/mL, respectively. Further analysis elucidated that the anti-inflammatory activity of compound 10 might be exerted through inhibiting the phosphorylation of ERK1/2 and JNK in MAPK signaling pathways to decrease NO and pro-inflammatory cytokines production.ConclusionsOur results indicated that 4′-O-Demethylophiopogonanone E can be considered as a potential source of therapeutic medicine for inflammatory diseases.

【 授权许可】

CC BY   
© The Author(s). 2017