Essential oil from Xylopia frutescens Aubl. reduces cytosolic calcium levels on guinea pig ileum: mechanism underlying its spasmolytic potential

BMC Complementary and Alternative Medicine

Research Article

Layanne Cabral da Cunha Araujo¹  Maria da Conceição Correia Silva²  Luiz Henrique César Vasconcelos²  Iara Leão Luna de Souza²  Bagnólia Araújo da Silva³  Josean Fechine Tavares³  Fabiana de Andrade Cavalcante⁴  Edgar Julian Paredes-Gamero⁵  Ana Carolina de Carvalho Correia⁶  Vicente Carlos de Oliveira Costa⁷ 

[1]Centro de Ciências Exatas e da Natureza/Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil

[2]Centro de Ciências da Saúde/Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil

[3]Centro de Ciências da Saúde/Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil

[4]Instituto de Pesquisa em Fármacos e Medicamentos/Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil

[5]Centro de Ciências da Saúde/Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil

[6]Instituto de Pesquisa em Fármacos e Medicamentos/Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil

[7]Universidade Federal da Paraíba, Centro de Ciências da Saúde/Pós-Graduação em Produtos Naturais e Sintéticos Bioativos/Laboratório de Farmacologia Funcional Prof. George Thomas, Cidade, Universitária, P.O. Box 5009, 58051-970, João Pessoa, Paraíba, Brazil

[8]Instituto Nacional de Farmacologia/Universidade Federal de São Paulo, São Paulo, São Paulo, Brazil

[9]Instituto de Ciências Biológicas e da Saúde/Universidade Federal de Alagoas, Maceió, Alagoas, Brazil

[10]Instituto de Pesquisa em Fármacos e Medicamentos/Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil

关键词: Xylopia frutescens;    Essential oil;    Spasmolytic action;    Guinea pig ileum;    Calcium;   

DOI  :  10.1186/s12906-015-0849-3

 received in 2015-03-26, accepted in 2015-09-03,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundXylopia frutescens Aubl. (embira, semente-de-embira or embira-vermelha), is used in folk medicine as antidiarrheal. The essential oil from its leaves (XF-EO) has been found to cause smooth muscle relaxation. Thus, the aim of this study was to investigate the spasmolytic action by which XF-EO acts on guinea pig ileum.MethodsThe components of the XF-EO were identified by gas chromatography-mass spectrometry. Segments of guinea pig ileum were suspended in organ bath containing modified Krebs solution at 37 °C, bubbled with carbogen mixture under a resting tension of 1 g. Isotonic contractions were registered using kymographs and isometric contractions using force transducer coupled to an amplifier and computer. Fluorescence measurements were obtained with a microplate reader using Fluo-4.ResultsForty-three constituents were identified in XF-EO, mostly mono- and sesquiterpenes. XF-EO has been found to cause relaxation on guinea pig ileum. The essential oil inhibited in a concentration-dependent manner both CCh- and histamine-induced phasic contractions, being more potent on histamine-induced contractions as well as antagonized histamine-induced cumulative contractions in a non-competitive antagonism profile. XF-EO relaxed in a concentration-dependent manner the ileum pre-contracted with KCl and histamine. Since the potency was smaller in organ pre-contracted with KCl, it was hypothesized that XF-OE would be acting as a K+ channel positive modulator. In the presence of CsCl (non-selective K+ channel blocker), the relaxant potency of XF-OE was not altered, indicating a non-participation of these channels. Moreover, XF-EO inhibited CaCl2-induced cumulative contractions in a depolarizing medium nominally without Ca2+ and relaxed the ileum pre-contracted with S-(-)-Bay K8644 in a concentration-dependent manner, thus, was confirmed the inhibition of Ca2+ influx through Cav1 by XF-EO. In cellular experiments, the viability of longitudinal layer myocytes from guinea pig ileum was not altered in the presence of XF-OE and the Fluo-4-associated fluorescence intensity in these intestinal myocytes stimulated by histamine was reduced by the essential oil, indicating a [Ca2+]c reduction.ConclusionSpasmolytic action mechanism of XF-EO on guinea pig ileum can involve histaminergic receptor antagonism and Ca2+ influx blockade, which results in [Ca2+]c reduction leading to smooth muscle relaxation.

【 授权许可】

CC BY   
© Souza et al. 2015