Chitosan in viscosupplementation: in vivo effect on rabbit subchondral bone

BMC Musculoskeletal Disorders

Research Article

R. Rieger¹  T. Hoc¹  S. Kaderli²  C. Boulocher³ 

[1] LTDS, UMR CNRS 5513, Université de Lyon, Ecole Centrale de Lyon, 36 av. Guy de Collongue, 69134, Ecully Cedex, France;School of Pharmaceutical Sciences, University of Geneva and University of Lausanne, Quai Ernest-Ansermet 30, 1211, Geneva, Switzerland;VetAgro Sup, University of Lyon, Veterinary Campus of VetAgro Sup, 69280, Marcy l’Etoile, France;

关键词: ACLT-induced osteoarthritis;    Hyaluronic acid;    Chitosan;    Subchondral bone;    Microarchitecture;    Mineral density;   

DOI  :  10.1186/s12891-017-1700-4

 received in 2017-01-16, accepted in 2017-07-27,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundTo investigate the effect of intra-articular injection of Chitosan (Cs) added to hyaluronic acid (HA) on subchondral bone during osteoarthritis (OA), microarchitectural parameters and mineral density were measured in a rabbit model of early OA. A novel hybrid hydrogel adding reacetylated Cs of fungal origin to HA was compared to high molecular weight HA commercial formulation.MethodEighteen rabbits underwent unilateral anterior cruciate ligament transection (ACLT) and were divided into three groups (Saline-group, HA-group and Hybrid-group) depending on the intra-articular injection compound. Eight contralateral knees were used as non-operated controls (Contralateral-group). Micro-computed tomography was performed six weeks post-ACLT to study subchondral bone microarchitectural parameters and mineral density at an early stage of OA development.ResultsCartilage thickness mean value was reduced only in Saline-group compared to Contralateral-group. When the Hybrid-group was compared to Saline-group, subchondral bone microarchitectural parameters (trabecular thickness and trabecular bone volume fraction) were significantly changed; subchondral bone plate and trabecular bone mineral densities (bone mineral density and tissue mineral density) were reduced. When the Hybrid-group was compared to HA-group, subchondral bone microarchitectural parameters (subchondral plate thickness and trabecular thickness) and trabecular bone mineral densities (bone mineral density and tissue mineral density) were significantly decreased.ConclusionConclusion: Compared to HA alone, the novel hybrid hydrogel, constituted of Cs added to HA, enhanced microarchitectural parameters and mineral density changes, leading to subchondral bone loss in a rabbit model of early experimental OA.

【 授权许可】

CC BY   
© The Author(s). 2017