Partially purified components of Uncaria sinensis attenuate blood brain barrier disruption after ischemic brain injury in mice

BMC Complementary and Alternative Medicine

Research Article

Young Whan Choi¹  Jin Woo Hong²  Hyung Bum Seo³  Bo Kyung Kang³  Ji Hyun Kim³  Byung Tae Choi⁴  Hwa Kyoung Shin⁴ 

[1] Department of Horticultural Bioscience, College of Natural Resource and Life Science, Pusan National University, 626-706, Miryang, Gyeongnam, Republic of Korea;Division of Clinical Medicine 1, School of Korean Medicine, Pusan National University, 626-870, Yangsan, Gyeongnam, Republic of Korea;Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University, 626-870, Yangsan, Gyeongnam, Republic of Korea;Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University, 626-870, Yangsan, Gyeongnam, Republic of Korea;Korean Medical Science Research Center for Healthy-Aging, Pusan National University, 626-870, Yangsan, Gyeongnam, Republic of Korea;

关键词: Blood–brain barrier;    Focal cerebral ischemia;    MMP;    Tight junction protein;    Uncaria sinensis;   

DOI  :  10.1186/s12906-015-0678-4

 received in 2014-12-13, accepted in 2015-05-19,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundUncaria sinensis (US) has long been used in traditional Korean medicine to relieve various nervous-related symptoms and cardiovascular disease. We recently showed the neuroprotective and cerebrovascular protective effects of US on cerebral ischemia; however, its effects on the blood–brain barrier (BBB) are poorly understood. In this study, the effects of partially purified components of US (PPUS) on BBB disruption were investigated in mice subjected to ischemic brain injury.MethodsFocal cerebral ischemia was induced in C57BL/6J mice by photothrombotic cortical ischemia. PPUS was injected intraperitoneally 30 min before ischemic insults. Infarct volume, neurological score, wire-grip test, Evans blue leakage and brain water content were then examined 24 h after ischemic brain injury.ResultsInfarct volume was significantly reduced and neurological deficit and motor deficit were greatly improved in PPUS-pretreated mice relative to those treated with vehicle following photothrombotic cortical ischemia. Brain edema-induced change of Evans blue extravasation and water content in the ipsilateral hemisphere were alleviated by treatment with PPUS. In addition, PPUS significantly reduced ischemic brain injury-induced degradation of tight junction proteins and elevation of matrix metalloproteinase-9 (MMP-9).ConclusionsPPUS prevents cerebral ischemic damage by BBB protection, and these effects were associated with inhibition of tight junction degradation and MMP-9 induction.

【 授权许可】

Unknown   
© Seo et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.