Partially purified components of Uncaria sinensis attenuate blood brain barrier disruption after ischemic brain injury in mice

BMC Complementary and Alternative Medicine

Hwa Kyoung Shin²  Byung Tae Choi²  Jin Woo Hong⁴  Young Whan Choi³  Ji Hyun Kim¹  Bo Kyung Kang¹  Hyung Bum Seo¹ 

[1] Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University, Yangsan 626-870, Gyeongnam, Republic of Korea;Korean Medical Science Research Center for Healthy-Aging, Pusan National University, Yangsan 626-870, Gyeongnam, Republic of Korea;Department of Horticultural Bioscience, College of Natural Resource and Life Science, Pusan National University, Miryang 626-706, Gyeongnam, Republic of Korea;Division of Clinical Medicine 1, School of Korean Medicine, Pusan National University, Yangsan 626-870, Gyeongnam, Republic of Korea

关键词: Uncaria sinensis;    Tight junction protein;    MMP;    Focal cerebral ischemia;    Blood–brain barrier;   

Others  :  1210268
DOI  :  10.1186/s12906-015-0678-4

 received in 2014-12-13, accepted in 2015-05-19,  发布年份 2015

【 摘 要 】

Background

Uncaria sinensis (US) has long been used in traditional Korean medicine to relieve various nervous-related symptoms and cardiovascular disease. We recently showed the neuroprotective and cerebrovascular protective effects of US on cerebral ischemia; however, its effects on the blood–brain barrier (BBB) are poorly understood. In this study, the effects of partially purified components of US (PPUS) on BBB disruption were investigated in mice subjected to ischemic brain injury.

Methods

Focal cerebral ischemia was induced in C57BL/6J mice by photothrombotic cortical ischemia. PPUS was injected intraperitoneally 30 min before ischemic insults. Infarct volume, neurological score, wire-grip test, Evans blue leakage and brain water content were then examined 24 h after ischemic brain injury.

Results

Infarct volume was significantly reduced and neurological deficit and motor deficit were greatly improved in PPUS-pretreated mice relative to those treated with vehicle following photothrombotic cortical ischemia. Brain edema-induced change of Evans blue extravasation and water content in the ipsilateral hemisphere were alleviated by treatment with PPUS. In addition, PPUS significantly reduced ischemic brain injury-induced degradation of tight junction proteins and elevation of matrix metalloproteinase-9 (MMP-9).

Conclusions

PPUS prevents cerebral ischemic damage by BBB protection, and these effects were associated with inhibition of tight junction degradation and MMP-9 induction.

【 授权许可】

   
2015 Seo et al.; licensee BioMed Central.

【 图 表 】

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