Dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated Streptococcus lutetiensis

BMC Microbiology

Research Article

Zhemin Zhou¹  Lei Wang¹  Marcelo Gottschalk²  Lianqing Li³  Shan Lu⁴  Huaiqi Jing⁴  Yanwen Xiong⁴  Pengcheng Du⁴  Dong Jin⁴  Xuemei Bai⁴  Hui Sun⁴  Han Zheng⁴  Changyun Ye⁴  Haiyin Wang⁴  Zhenjun Li⁴  Chen Chen⁴  Yanmei Xu⁴  Jianguo Xu⁴ 

[1]College of Life Sciences, Nankai University, Tianjin, China

[2]Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de médecine vétérinaire, Université de Montréal, Québec, Canada

[3]Molecular Microbiology Laboratory, ShanXi Children’s Hospital, 030013, TaiYuan, China

[4]State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China

关键词: Microbial communities;    16S rRNA gene analysis;    Streptococcus lutetiensis;    Genome analysis;    Pathogenic island;   

DOI  :  10.1186/1471-2180-13-141

 received in 2012-11-19, accepted in 2013-06-10,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe sequences of the 16S rRNA genes extracted from fecal samples provide insights into the dynamics of fecal microflora. This potentially gives valuable etiological information for patients whose conditions have been ascribed to unknown pathogens, which cannot be accomplished using routine culture methods. We studied 33 children with diarrhea who were admitted to the Children’s Hospital in Shanxi Province during 2006.ResultsNineteen of 33 children with diarrhea could not be etiologically diagnosed by routine culture and polymerase chain reaction methods. Eleven of 19 children with diarrhea of unknown etiology had Streptococcus as the most dominant fecal bacterial genus at admission. Eight of nine children whom three consecutive fecal samples were collected had Streptococcus as the dominant fecal bacterial genus, including three in the Streptococcus bovis group and three Streptococcus sp., which was reduced during and after recovery. We isolated strains that were possibly from the S. bovis group from feces sampled at admission, which were then identified as Streptococcus lutetiensis from one child and Streptococcus gallolyticus subsp. pasteurianus from two children. We sequenced the genome of S. lutetiensis and identified five antibiotic islands, two pathogenicity islands, and five unique genomic islands. The identified virulence genes included hemolytic toxin cylZ of Streptococcus agalactiae and sortase associated with colonization of pathogenic streptococci.ConclusionsWe identified S. lutetiensis and S. gallolyticus subsp. pasteurianus from children with diarrhea of unknown etiology, and found pathogenic islands and virulence genes in the genome of S. lutetiensis.

【 授权许可】

Unknown   
© Jin et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.