Dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated Streptococcus lutetiensis

BMC Microbiology

Jianguo Xu³  Marcelo Gottschalk⁴  Changyun Ye³  Lei Wang²  Hui Sun³  Xuemei Bai³  Han Zheng³  Yanwen Xiong³  Haiyin Wang³  Pengcheng Du³  Yanmei Xu³  Huaiqi Jing³  Zhemin Zhou²  Zhenjun Li³  Shan Lu³  Lianqing Li¹  Chen Chen³  Dong Jin³ 

[1]Molecular Microbiology Laboratory, ShanXi Children’s Hospital, TaiYuan, 030013, China

[2]College of Life Sciences, Nankai University, Tianjin, China

[3]State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China

[4]Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de médecine vétérinaire, Université de Montréal, Québec, Canada

关键词: Pathogenic island;    Genome analysis;    Streptococcus lutetiensis;    16S rRNA gene analysis;    Microbial communities;   

Others  :  1143616
DOI  :  10.1186/1471-2180-13-141

 received in 2012-11-19, accepted in 2013-06-10,  发布年份 2013

【 摘 要 】

Background

The sequences of the 16S rRNA genes extracted from fecal samples provide insights into the dynamics of fecal microflora. This potentially gives valuable etiological information for patients whose conditions have been ascribed to unknown pathogens, which cannot be accomplished using routine culture methods. We studied 33 children with diarrhea who were admitted to the Children’s Hospital in Shanxi Province during 2006.

Results

Nineteen of 33 children with diarrhea could not be etiologically diagnosed by routine culture and polymerase chain reaction methods. Eleven of 19 children with diarrhea of unknown etiology had Streptococcus as the most dominant fecal bacterial genus at admission. Eight of nine children whom three consecutive fecal samples were collected had Streptococcus as the dominant fecal bacterial genus, including three in the Streptococcus bovis group and three Streptococcus sp., which was reduced during and after recovery. We isolated strains that were possibly from the S. bovis group from feces sampled at admission, which were then identified as Streptococcus lutetiensis from one child and Streptococcus gallolyticus subsp. pasteurianus from two children. We sequenced the genome of S. lutetiensis and identified five antibiotic islands, two pathogenicity islands, and five unique genomic islands. The identified virulence genes included hemolytic toxin cylZ of Streptococcus agalactiae and sortase associated with colonization of pathogenic streptococci.

Conclusions

We identified S. lutetiensis and S. gallolyticus subsp. pasteurianus from children with diarrhea of unknown etiology, and found pathogenic islands and virulence genes in the genome of S. lutetiensis.

【 授权许可】

   
2013 Jin et al.; licensee BioMed Central Ltd.

【 图 表 】

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