Establishment and characterization of a new human pancreatic adenocarcinoma cell line with high metastatic potential to the lung

BMC Cancer

Research Article

Thomas Streichert¹  Benjamin Otto¹  Daniel Wicklein¹  Sabrina Thieltges²  Maximilian Bockhorn²  Tatyana Kalinina²  Cenap Güngör²  Viacheslav Kalinin²  Emre F Yekebas²  Maren Möller-Krull²  Jakob R Izbicki²  Katharina E Effenberger³  Judith Dierlamm⁴  Eva Maria Murga Penas⁴  Udo Schumacher⁵  Ronald Simon⁶  Heidi Schwarzenbach⁷ 

[1] Department of Clinical Chemistry, University Hospital Hamburg, Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany;Department of General, Visceral and Thoracic Surgery, University Hospital Hamburg, Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany;Department of General, Visceral and Thoracic Surgery, University Hospital Hamburg, Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany;Institute of Tumor Biology, University Hospital Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany;Hubertus Wald Tumorzentrum, University Cancer Center Hamburg, University Medical Center, University Hospital Hamburg, Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany;Institute of Anatomy and Experimental Morphology, University Hospital Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany;Institute of Pathology, University Hospital Hamburg, Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany;Institute of Tumor Biology, University Hospital Hamburg-Eppendorf, Martinistrasse 52, 20246, Hamburg, Germany;

关键词: Pancreatic Cancer;    Gemcitabine;    Cetuximab;    Gefitinib;    Single Nucleotide Polymorphism Array;   

DOI  :  10.1186/1471-2407-10-295

 received in 2009-11-20, accepted in 2010-06-16,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundPancreatic cancer is still associated with devastating prognosis. Real progress in treatment options has still not been achieved. Therefore new models are urgently needed to investigate this deadly disease. As a part of this process we have established and characterized a new human pancreatic cancer cell line.MethodsThe newly established pancreatic cancer cell line PaCa 5061 was characterized for its morphology, growth rate, chromosomal analysis and mutational analysis of the K-ras, EGFR and p53 genes. Gene-amplification and RNA expression profiles were obtained using an Affymetrix microarray, and overexpression was validated by IHC analysis. Tumorigenicity and spontaneous metastasis formation of PaCa 5061 cells were analyzed in pfp-/-/rag2-/- mice. Sensitivity towards chemotherapy was analysed by MTT assay.ResultsPaCa 5061 cells grew as an adhering monolayer with a doubling time ranging from 30 to 48 hours. M-FISH analyses showed a hypertriploid complex karyotype with multiple numerical and unbalanced structural aberrations. Numerous genes were overexpressed, some of which have previously been implicated in pancreatic adenocarcinoma (GATA6, IGFBP3, IGFBP6), while others were detected for the first time (MEMO1, RIOK3). Specifically highly overexpressed genes (fold change > 10) were identified as EGFR, MUC4, CEACAM1, CEACAM5 and CEACAM6. Subcutaneous transplantation of PaCa 5061 into pfp-/-/rag2-/- mice resulted in formation of primary tumors and spontaneous lung metastasis.ConclusionThe established PaCa 5061 cell line and its injection into pfp-/-/rag2-/- mice can be used as a new model for studying various aspects of the biology of human pancreatic cancer and potential treatment approaches for the disease.

【 授权许可】

CC BY   
© Kalinina et al; licensee BioMed Central Ltd. 2010