Mutations in epidermal growth factor receptor and K-ras in Chinese patients with colorectal cancer

BMC Medical Genetics

Research Article

Li Jin¹  Zuo Yunxia¹  Cao Jun¹  Zhou Xueke¹  Lu Yachao²  Zhu Guanshan² 

[1] Department of Medical Oncology, Fudan University Cancer Hospital, Shanghai Medical School, 200032, Shanghai, P.R. China;Innovation Center China, AstraZeneca Global R&D, 898 Halei Road, 201203, Shanghai, P.R. China;

关键词: Overall Survival;    Epidermal Growth Factor Receptor;    Cetuximab;    Gefitinib;    Epidermal Growth Factor Receptor Mutation;   

DOI  :  10.1186/1471-2350-11-34

 received in 2009-08-11, accepted in 2010-02-26,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundMutations of EGFR and K-ras are biomarkers for predicting the efficacy of targeting agents in non-small-cell lung cancer (NSCLC) and colorectal cancer (CRC). Data on the gene mutation status of EGFR and K-ras in Chinese patients with CRC are limited.MethodsEGFR mutations in exon 18-21 and K-ras mutations in exon 1 and 2 were detected in tumor samples from 101 Chinese patients with CRC by polymerase chain reaction-single strand conformational polymorphism. The relationship between patients' characteristics and survival time and gene mutation status were analyzed using the Statistical Package for the Social Sciences.ResultsOnly two samples (2.0%) had EGFR mutations in exon 18 or 21, and 33 of 101 samples (32.7%) had K-ras mutations in codon 12, 13, 45, 69, or 80. Univariate analysis suggested that differentiation might be correlated with K-ras mutations (p = 0.05), which was confirmed by a logistic regression model (p = 0.04). The median overall survival (OS) and median survival after metastasis were 44.0 and 18.0 months, respectively, in the mutant K-ras group, and 53.3 and 19.0 months, respectively, in the wild K-ras group. K-ras mutation was not an independent prognostic factor for OS or survival after metastasis (p = 0.79 and 0.78, respectively).ConclusionsIn Chinese patients with CRC, EGFR mutations were rare, and K-ras mutations were similar to those of Europeans. New mutations in codons 45, 69, and 80 were found in the Chinese population. Poor differentiation was an independent factor related to K-ras mutations.

【 授权许可】

Unknown   
© Yunxia et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.