Diverse set of microRNAs are responsive to powdery mildew infection and heat stress in wheat (Triticum aestivum L.)

BMC Plant Biology

Research Article

Mingming Xin¹  Yingyin Yao¹  Yu Wang¹  Huiru Peng¹  Zhongfu Ni¹  Chaojie Xie²  Qixin Sun² 

[1] State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100094, Beijing, China;National Plant Gene Research Centre (Beijing), 100094, Beijing, China;State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100094, Beijing, China;National Plant Gene Research Centre (Beijing), 100094, Beijing, China;Department of Plant Genetics & Breeding, China Agricultural University, Yuanmingyuan Xi Road No. 2, 100193, Haidian District, Beijing, China;

关键词: Heat Stress;    Powdery Mildew;    miRNA Family;    Chinese Spring;    Brachypodium Distachyon;   

DOI  :  10.1186/1471-2229-10-123

 received in 2009-07-10, accepted in 2010-06-24,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundMicroRNAs (miRNAs) are a class of small non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition. MiRNAs can have large-scale regulatory effects on development and stress response in plants.ResultsTo test whether miRNAs play roles in regulating response to powdery mildew infection and heat stress in wheat, by using Solexa high-throughput sequencing we cloned the small RNA from wheat leaves infected by preponderant physiological strain Erysiphe graminis f. sp. tritici (Egt) or by heat stress treatment. A total of 153 miRNAs were identified, which belong to 51 known and 81 novel miRNA families. We found that 24 and 12 miRNAs were responsive to powdery mildew infection and heat stress, respectively. We further predicted that 149 target genes were potentially regulated by the novel wheat miRNA.ConclusionsOur results indicated that diverse set of wheat miRNAs were responsive to powdery mildew infection and heat stress and could function in wheat responses to both biotic and abiotic stresses.

【 授权许可】

Unknown   
© Xin et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.