Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing

BMC Plant Biology

Research Article

Mingming Xin¹  Na Song¹  Yingyin Yao¹  Chaojie Xie¹  Yu Wang¹  Dandan Qin¹  Huiru Peng¹  Zhaorong Hu¹  Zhongfu Ni¹  Qixin Sun² 

[1] State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100094, Beijing, PR China;National Plant Gene Research Centre (Beijing), 100094, Beijing, PR China;State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100094, Beijing, PR China;National Plant Gene Research Centre (Beijing), 100094, Beijing, PR China;Department of Plant Genetics & Breeding, China Agricultural University, Yuanmingyuan Xi Road No. 2, 100193, Haidian District, Beijing, PR;

关键词: Heat Stress;    Powdery Mildew;    miRNA Precursor;    Signal Recognition Particle;    Powdery Mildew Infection;   

DOI  :  10.1186/1471-2229-11-61

 received in 2010-10-21, accepted in 2011-04-07,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundBiotic and abiotic stresses, such as powdery mildew infection and high temperature, are important limiting factors for yield and grain quality in wheat production. Emerging evidences suggest that long non-protein coding RNAs (npcRNAs) are developmentally regulated and play roles in development and stress responses of plants. However, identification of long npcRNAs is limited to a few plant species, such as Arabidopsis, rice and maize, no systematic identification of long npcRNAs and their responses to abiotic and biotic stresses is reported in wheat.ResultsIn this study, by using computational analysis and experimental approach we identified 125 putative wheat stress responsive long npcRNAs, which are not conserved among plant species. Among them, some were precursors of small RNAs such as microRNAs and siRNAs, two long npcRNAs were identified as signal recognition particle (SRP) 7S RNA variants, and three were characterized as U3 snoRNAs. We found that wheat long npcRNAs showed tissue dependent expression patterns and were responsive to powdery mildew infection and heat stress.ConclusionOur results indicated that diverse sets of wheat long npcRNAs were responsive to powdery mildew infection and heat stress, and could function in wheat responses to both biotic and abiotic stresses, which provided a starting point to understand their functions and regulatory mechanisms in the future.

【 授权许可】

Unknown   
© Xin et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.