Perineural pretreatment of bee venom attenuated the development of allodynia in the spinal nerve ligation injured neuropathic pain model; an experimental study

BMC Complementary and Alternative Medicine

Research Article

Seong Soo Choi¹  Won Uk Koh¹  Jun Gol Song¹  Jin Woo Shin¹  Sun Kyung Lee¹  Jong Hyuk Lee¹  So Hee Lee¹  Jeong Gil Leem¹  Yoon Kyung Lee² 

[1] Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan, College of Medicine, 388-1, Pungnap-2Dong, Songpa-Gu, Seoul, South Korea;Department of Anesthesiology and Pain Medicine, Hangang Sacred Heart Hospital, Hallym University Medical Center, Youngdeungpo-Dong, Youngdeungpo-Gu, Seoul, South Korea;

关键词: Allodynia;    Bee venom;    Neuropathic pain;    Transient receptor potential;   

DOI  :  10.1186/1472-6882-14-431

 received in 2014-06-27, accepted in 2014-10-28,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDiluted bee venom (BV) is known to have anti-nociceptive and anti-inflammatory effects. We therefore assessed whether perineural bee venom pretreatment could attenuate the development of neuropathic pain in the spinal nerve ligation injured animal model.MethodsNeuropathic pain was surgically induced in 30 male Sprague Dawley rats by ligation of the L5 and L6 spinal nerves, with 10 rats each treated with saline and 0.05 and 0.1 mg BV. Behavioral testing for mechanical, cold, and thermal allodynia was conducted on postoperative days 3 to 29. Three rats in each group and 9 sham operated rats were sacrificed on day 9, and the expression of transient receptor potential vanilloid type 1 (TRPV1), ankyrin type 1 (TRPA1), and melastatin type 8 (TRPM8) receptors in the ipsilateral L5 dorsal root ganglion was analyzed.ResultsThe perineural administration of BV to the spinal nerves attenuated the development of mechanical, thermal, and cold allodynia, and the BV pretreatment reduced the expression of TRPV1, TRPA1, TRPM8 and c − Fos in the ipsilateral dorsal root ganglion.ConclusionThe current study demonstrates that the perineural pretreatment with diluted bee venom before the induction of spinal nerve ligation significantly suppresses the development of neuropathic pain. Furthermore, this bee venom induced suppression was strongly related with the involvement of transient receptor potential family members.

【 授权许可】

Unknown   
© Koh et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.