Perineural pretreatment of bee venom attenuated the development of allodynia in the spinal nerve ligation injured neuropathic pain model; an experimental study

BMC Complementary and Alternative Medicine

Jin Woo Shin²  Jun Gol Song²  Jeong Gil Leem²  Yoon Kyung Lee¹  Sun Kyung Lee²  So Hee Lee²  Jong Hyuk Lee²  Seong Soo Choi²  Won Uk Koh² 

[1] Department of Anesthesiology and Pain Medicine, Hangang Sacred Heart Hospital, Hallym University Medical Center, Youngdeungpo-Dong, Youngdeungpo-Gu, Seoul, Korea;Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan, College of Medicine, Pungnap-2Dong, Songpa-Gu, Seoul 388-1, Korea

关键词: Transient receptor potential;    Neuropathic pain;    Bee venom;    Allodynia;   

Others  :  1085637
DOI  :  10.1186/1472-6882-14-431

 received in 2014-06-27, accepted in 2014-10-28,  发布年份 2014

【 摘 要 】

Background

Diluted bee venom (BV) is known to have anti-nociceptive and anti-inflammatory effects. We therefore assessed whether perineural bee venom pretreatment could attenuate the development of neuropathic pain in the spinal nerve ligation injured animal model.

Methods

Neuropathic pain was surgically induced in 30 male Sprague Dawley rats by ligation of the L5 and L6 spinal nerves, with 10 rats each treated with saline and 0.05 and 0.1 mg BV. Behavioral testing for mechanical, cold, and thermal allodynia was conducted on postoperative days 3 to 29. Three rats in each group and 9 sham operated rats were sacrificed on day 9, and the expression of transient receptor potential vanilloid type 1 (TRPV1), ankyrin type 1 (TRPA1), and melastatin type 8 (TRPM8) receptors in the ipsilateral L5 dorsal root ganglion was analyzed.

Results

The perineural administration of BV to the spinal nerves attenuated the development of mechanical, thermal, and cold allodynia, and the BV pretreatment reduced the expression of TRPV1, TRPA1, TRPM8 and c − Fos in the ipsilateral dorsal root ganglion.

Conclusion

The current study demonstrates that the perineural pretreatment with diluted bee venom before the induction of spinal nerve ligation significantly suppresses the development of neuropathic pain. Furthermore, this bee venom induced suppression was strongly related with the involvement of transient receptor potential family members.

【 授权许可】

   
2014 Koh et al.; licensee BioMed Central Ltd.

【 图 表 】

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