High-resolution mapping and characterization of qRgls2, a major quantitative trait locus involved in maize resistance to gray leaf spot

BMC Plant Biology

Research Article

Siquan Shao¹  Wei Chen²  Xingming Fan²  Jing Tan²  Yan Zhang³  Tao Zhong³  Ling Xu³  Mang Zhu³  Mingliang Xu³ 

[1] Baoshan Institute of Agricultural Science, Taibao North Road, Longyang District, 678000, Baoshan, PR China;Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Longtou Street, 650205, Kunming, PR China;National Maize Improvement Center of China, China Agricultural University, No.2 Yuanmingyuan West Road, 100193, Beijing, PR China;

关键词: Maize;    GLS;    QTL;    Fine-mapping;    Candidate genes;   

DOI  :  10.1186/s12870-014-0230-6

 received in 2014-03-08, accepted in 2014-08-18,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundGray leaf spot (GLS) caused by Cercospora zeae-maydis (Czm) or Cercospora zeina (Cz) is a devastating maize disease and results in substantial yield reductions worldwide. GLS resistance is a quantitatively inherited trait. The development and cultivation of GLS-resistant maize hybrids are the most cost-effective and efficient ways to control this disease.ResultsWe previously detected a major GLS resistance QTL, qRgls2, in bin 5.03-04, which spans the whole centromere of chromosome 5 encompassing a physical distance of ~110-Mb. With advanced backcross populations derived from the cross between the resistant Y32 and susceptible Q11 inbred lines, a sequential recombinant-derived progeny testing strategy was adapted to fine map qRgls2. We narrowed the region of qRgls2 from an initial ~110-Mb to an interval of ~1-Mb, flanked by the markers G346 and DD11. qRgls2 showed predominantly additive genetic effects and significantly increased the resistance percentage by 20.6 to 24.6% across multiple generations. A total of 15 genes were predicted in the mapped region according to the 5b.60 annotation of the maize B73 genome v2. Two pieces of the mapped qRgls2 region shared collinearity with two distant segments on maize chromosome 4.ConclusionsqRgls2, a major QTL involved in GLS resistance, was mapped to a ~1-Mb region close to the centromere of chromosome 5. There are 15 predicted genes in the mapped region. It is assumed that qRgls2 could be widely used to improve maize resistance to GLS.

【 授权许可】

CC BY   
© Xu et al.; licensee BioMed Central Ltd. 2014