Combined linkage and association mapping reveals candidates for Scmv1, a major locus involved in resistance to sugarcane mosaic virus (SCMV) in maize

BMC Plant Biology

Research Article

Thomas Lübberstedt¹  Ursula Karoline Frei¹  Yongfu Tao²  Lu Jiang²  Mingliang Xu²  Baobao Wang²  Jinsheng Lai²  Yan Zhang²  Rui Zhang²  Qingqing Liu²  Christina Roenn Ingvardsen³ 

[1] Department of Agronomy, Iowa State University, 1204 Agronomy Hall, 50011, Ames, Iowa, USA;National Maize Improvement Center, China Agricultural University, 2 West Yuanmingyuan Road, 100193, Beijing, People’s Republic of China;Research Center Flakkebjerg, University of Aarhus, 4200, Slagelse, Denmark;

关键词: Maize;    SCMV;    QTL;    Fine-mapping;    Association mapping;   

DOI  :  10.1186/1471-2229-13-162

 received in 2013-08-01, accepted in 2013-10-09,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundSugarcane mosaic virus (SCMV) disease causes substantial losses of grain yield and forage biomass in susceptible maize cultivars. Maize resistance to SCMV is associated with two dominant genes, Scmv1 and Scmv2, which are located on the short arm of chromosome 6 and near the centromere region of chromosome 3, respectively. We combined both linkage and association mapping to identify positional candidate genes for Scmv1.ResultsScmv1 was fine-mapped in a segregating population derived from near-isogenic lines and further validated and fine-mapped using two recombinant inbred line populations. The combined results assigned the Scmv1 locus to a 59.21-kb interval, and candidate genes within this region were predicted based on the publicly available B73 sequence. None of three predicted genes that are possibly involved in the disease resistance response are similar to receptor-like resistance genes. Candidate gene–based association mapping was conducted using a panel of 94 inbred lines with variable resistance to SCMV. A presence/absence variation (PAV) in the Scmv1 region and two polymorphic sites around the Zmtrx-h gene were significantly associated with SCMV resistance.ConclusionCombined linkage and association mapping pinpoints Zmtrx-h as the most likely positional candidate gene for Scmv1. These results pave the way towards cloning of Scmv1 and facilitate marker-assisted selection for potyvirus resistance in maize.

【 授权许可】

Unknown   
© Tao et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.