Abnormal fecal microbiota community and functions in patients with hepatitis B liver cirrhosis as revealed by a metagenomic approach

BMC Gastroenterology

Research Article

Xuesong Wang¹  Liuyu Huang¹  Jing Yuan¹  Wei Liu¹  Xiao Wei¹  Xiabei Yan¹  Xuelian Li¹  Zhan Yang¹  Dayang Zou¹  Simiao Wang¹  Juqiang Han² 

[1] Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda street, Fengtai District, 100071, Beijing, China;Institute of Hepatology, Beijing Military General Hospital, No. 5 Dongsishitiao South Gate Warehouse, Dongcheng District, 100700, Beijing, China;

关键词: Metagenome;    Cirrhosis;    Microbiota;    Metabolism;   

DOI  :  10.1186/1471-230X-13-175

 received in 2013-09-06, accepted in 2013-12-23,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAssessment and characterization of human colon microbiota is now a major research area in human diseases, including in patients with hepatitis B liver cirrhosis (HBLC).MethodsWe recruited 120 patients with HBLC and 120 healthy controls. The fecal microbial community and functions in the two groups were analyzed using high-throughput Solexa sequencing of the complete metagenomic DNA and bioinformatics methods.ResultsCommunity and metabolism-wide changes of the fecal microbiota in 20 HBLC patients and 20 healthy controls were observed and compared. A negative correlation was observed between the Child-Turcotte-Pugh scores and Bacteroidetes (P < 0.01), whereas a positive correlation was observed between the scores and Enterobacteriaceae and Veillonella (P < 0.01). Analysis of the additional 200 fecal microbiota samples demonstrated that these intestinal microbial markers might be useful for distinguishing liver cirrhosis microbiota samples from normal ones. The functional diversity was significantly reduced in the fecal microbiota of cirrhotic patients compared with in the controls. At the module or pathway levels, the fecal microbiota of the HBLC patients showed enrichment in the metabolism of glutathione, gluconeogenesis, branched-chain amino acid, nitrogen, and lipid (P < 0.05), whereas there was a decrease in the level of aromatic amino acid, bile acid and cell cycle related metabolism (P < 0.05).ConclusionsExtensive differences in the microbiota community and metabolic potential were detected in the fecal microbiota of cirrhotic patients. The intestinal microbial community may act as an independent organ to regulate the body’s metabolic balance, which may affect the prognosis for HBLC patients.

【 授权许可】

Unknown   
© Wei et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.