Abnormal fecal microbiota community and functions in patients with hepatitis B liver cirrhosis as revealed by a metagenomic approach

BMC Gastroenterology

Jing Yuan²  Liuyu Huang²  Juqiang Han¹  Xuelian Li²  Simiao Wang²  Wei Liu²  Xuesong Wang²  Zhan Yang²  Dayang Zou²  Xiabei Yan²  Xiao Wei² 

[1] Institute of Hepatology, Beijing Military General Hospital, No. 5 Dongsishitiao South Gate Warehouse, Dongcheng District, Beijing 100700, China;Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda street, Fengtai District, Beijing 100071, China

关键词: Metabolism;    Microbiota;    Cirrhosis;    Metagenome;   

Others  :  856782
DOI  :  10.1186/1471-230X-13-175

 received in 2013-09-06, accepted in 2013-12-23,  发布年份 2013

【 摘 要 】

Background

Assessment and characterization of human colon microbiota is now a major research area in human diseases, including in patients with hepatitis B liver cirrhosis (HBLC).

Methods

We recruited 120 patients with HBLC and 120 healthy controls. The fecal microbial community and functions in the two groups were analyzed using high-throughput Solexa sequencing of the complete metagenomic DNA and bioinformatics methods.

Results

Community and metabolism-wide changes of the fecal microbiota in 20 HBLC patients and 20 healthy controls were observed and compared. A negative correlation was observed between the Child-Turcotte-Pugh scores and Bacteroidetes (P < 0.01), whereas a positive correlation was observed between the scores and Enterobacteriaceae and Veillonella (P < 0.01). Analysis of the additional 200 fecal microbiota samples demonstrated that these intestinal microbial markers might be useful for distinguishing liver cirrhosis microbiota samples from normal ones. The functional diversity was significantly reduced in the fecal microbiota of cirrhotic patients compared with in the controls. At the module or pathway levels, the fecal microbiota of the HBLC patients showed enrichment in the metabolism of glutathione, gluconeogenesis, branched-chain amino acid, nitrogen, and lipid (P < 0.05), whereas there was a decrease in the level of aromatic amino acid, bile acid and cell cycle related metabolism (P < 0.05).

Conclusions

Extensive differences in the microbiota community and metabolic potential were detected in the fecal microbiota of cirrhotic patients. The intestinal microbial community may act as an independent organ to regulate the body’s metabolic balance, which may affect the prognosis for HBLC patients.

【 授权许可】

   
2013 Wei et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 3.

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