Anti-wrinkle effects of Seungma-Galgeun-Tang as evidenced by the inhibition of matrix metalloproteinase-I production and the promotion of type-1 procollagen synthesis

BMC Complementary and Alternative Medicine

Research Article

Hyang-Yu Kim¹  Chae Young Bang²  Se Young Choung³  Gwang Jun Yun⁴  Min Kyoung Kim⁵  Young Pyo Jang⁵ 

[1] Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, South Korea;Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University, Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, South Korea;Department of Preventive Pharmacy and Toxicology, College of Pharmacy, Kyung Hee University, Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, South Korea;Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, South Korea;Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, South Korea;Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, South Korea;Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Hoegi-dong, 130-701, Seoul, Dongdaemun-gu, South Korea;

关键词: Seungma-Galgeun-Tang;    Anti-wrinkle;    Matrix metalloproteinase-1;    Type-1 procollagen;   

DOI  :  10.1186/s12906-016-1095-z

 received in 2015-06-02, accepted in 2016-04-05,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundSeungma-Galgeun-Tang (SMGGT), a traditional herbal medicinal formula, has been used to treat various skin problems such as inflammation and rashes in Korean traditional medicine. In order to clarify the scientific evidence for the biological efficacy of SMGGT on the prevention of skin aging and in particular wrinkle formation, molecular anti-wrinkle parameters were evaluated in cultured human dermal fibroblasts.MethodsStandard SMGGT was prepared from KFDA-certified herbal medicines and the chemical fingerprint of SMGGT was verified by HPLC-ESI-MS to insure the quality of SMGGT. To evaluate the inhibitory effects of SMGGT on the synthesis of matrix metalloproteinase-1 (MMP-1) and type-1 procollagen, the content of MMP-1 and type-1 procollagen synthesizing enzymes in cultured human dermal fibroblasts were measured using an ELISA kit and Western Blot, respectively.ResultsThe treatment of SMGGT water extract significantly inhibited the production of MMP-1 and promoted type-1 procollagen synthesis concentration dependently.ConclusionsThese results suggest that SMGGT has the potential to prevent wrinkle formation by down-regulating MMP-1 and up-regulating type-1 procollagen in human dermal fibroblasts.

【 授权许可】

CC BY   
© Kim et al. 2016