OsACOS12, an orthologue of Arabidopsis acyl-CoA synthetase5, plays an important role in pollen exine formation and anther development in rice

BMC Plant Biology

Research Article

Yueling Li¹  Cheng Zhang¹  Jun Zhu¹  Shuangxi Xiong¹  Dandan Li¹  Qiangsheng Shi¹  Zhongnan Yang¹  Zongli Guo¹ 

[1] College of Life and Environment Sciences, Shanghai Normal University, 100 Guilin Road, 200234, Shanghai, China;

关键词: Oryza sativa;    OsACOS12;    Male sterility;    Pollen exine;    Anther cuticle;   

DOI  :  10.1186/s12870-016-0943-9

 received in 2016-08-21, accepted in 2016-11-03,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundSporopollenin is a major component of the pollen exine pattern. In Arabidopsis, acyl-CoA synthetase5 (ACOS5) is involved in sporopollenin precursor biosynthesis. In this study, we identified its orthologue, OsACOS12, in rice (Oryza sativa) and compared the functional conservation of ACOS in rice to Arabidopsis.ResultsSequence analysis showed that OsACOS12 shares 63.9 % amino acid sequence identity with ACOS5. The osacos12 mutation caused by a pre-mature stop codon in LOC_Os04g24530 exhibits defective sexine resulting in a male sterile phenotype in rice. In situ hybridization shows that OsACOS12 is expressed in tapetal cells and microspores at the transcript level. The localization of OsACOS12-GFP demonstrated that OsACOS12 protein is accumulated in tapetal cells and anther locules. OsACOS12 driven by the ACOS5 promoter could partially restore the male fertility of the acos5 mutant in Arabidopsis.ConclusionsOsACOS12 is an orthologue of ACOS5 that is essential for sporopollenin synthesis in rice. ACOS5 and OsACOS12 are conserved for pollen wall formation in monocot and dicot species.

【 授权许可】

CC BY   
© The Author(s). 2016