Response of turkey muscle satellite cells to thermal challenge. I. transcriptome effects in proliferating cells

BMC Genomics

Research Article

Sandra G. Velleman¹  Gale M. Strasburg²  Kent M. Reed³  Kristelle M. Mendoza³  Natalie E. Barnes³  Juan E. Abrahante⁴ 

[1] Department of Animal Sciences, The Ohio State University, Columbus, OH, USA;Ohio Agricultural Research and Development Center, Wooster, OH, USA;Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI, USA;Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN, USA;University of Minnesota Informatics Institute, University of Minnesota, Minneapolis, MN, USA;

关键词: Satellite cell;    Skeletal muscle;    Growth selected;    Turkey;    Proliferation;   

DOI  :  10.1186/s12864-017-3740-4

 received in 2017-01-23, accepted in 2017-04-27,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundClimate change poses a multi-dimensional threat to food and agricultural systems as a result of increased risk to animal growth, development, health, and food product quality. This study was designed to characterize transcriptional changes induced in turkey muscle satellite cells cultured under cold or hot thermal challenge to better define molecular mechanisms by which thermal stress alters breast muscle ultrastructure.ResultsSatellite cells isolated from the pectoralis major muscle of 7-weeks-old male turkeys from two breeding lines (16 weeks body weight-selected and it’s randombred control) were proliferated in culture at 33 °C, 38 °C or 43 °C for 72 h. Total RNA was isolated and 12 libraries subjected to RNAseq analysis. Statistically significant differences in gene expression were observed among treatments and between turkey lines with a greater number of genes altered by cold treatment than by hot and fewer differences observed between lines than between temperatures. Pathway analysis found that cold treatment resulted in an overrepresentation of genes involved in cell signaling/signal transduction and cell communication/cell signaling as compared to control (38 °C). Heat-treated muscle satellite cells showed greater tendency towards expression of genes related to muscle system development and differentiation.ConclusionsThis study demonstrates significant transcriptome effects on turkey skeletal muscle satellite cells exposed to thermal challenge. Additional effects on gene expression could be attributed to genetic selection for 16 weeks body weight (muscle mass). New targets are identified for further research on the differential control of satellite cell proliferation in poultry.

【 授权许可】

CC BY   
© The Author(s). 2017