Iridoids from Canthium subcordatum iso-butanol fraction with potent biological activities

BMC Complementary and Alternative Medicine

Research Article

Jean-de-Dieu Tamokou¹  Jules-Roger Kuiate¹  Christelle Joubouhi²  David Ngnokam²  Laurence Voutquenne-Nazabadioko³ 

[1] Department of Biochemistry, Laboratory of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon;Department of Chemistry, Laboratory of Environmental and Applied Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon;Groupe Isolement et Structure, Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312, Bat. 18 BP.1039, 51687, Reims cedex 2, France;

关键词: Canthium subcordatum;    Rubiaceae;    Fruit extracts;    Phytochemical analysis;    Iridoids;    Antimicrobial;    Antioxidant;   

DOI  :  10.1186/s12906-016-1536-8

 received in 2016-01-25, accepted in 2016-12-13,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe continuous emergence of multi-drug-resistant bacteria drastically reduces the efficacy of antibiotic armory and, consequently, increases the frequency of therapeutic failure. The discovery of new antibacterial drugs is an urgent need. The present study reports the antibacterial and antioxidant activities of the methanol extract, fractions and iridoids from Canthium subcordatum, a plant traditionally used as antidiabetic, anti-inflammatory, and antimicrobial.MethodsBroth microdilution assay was used to determine minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of extracts and iridoids against Staphylococcus aureus, Vibrio cholerae and Shigella flexneri. Antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and gallic acid equivalent antioxidant capacity (GAEAC) assays. The samples were also tested for their cytotoxicity against human red blood cells (RBC).ResultsThe methanol extract, hexane, ethyl acetate and iso-butanol fractions from C. subcordatum fruits displayed different degrees of antioxidant (EC50 = 62.83–70.17 μg/ml; GAEAC = 45.63–58.23 μg/ml) and antibacterial (MIC = 128–512 μg/ml) activities. Canthiumoside 1(1) and linearin (7) were the most active antioxidant (EC50 = 1.12–2.03 μg/ml; GAEAC = 79.82–92.35 μg/ml) and antibacterial (MIC = 8–64 μg/ml) compounds while the most sensitive bacterium was Staphylococcus aureus. The tested samples were non-toxic to normal cells.ConclusionOur results demonstrated that compounds 1 and 7 were potent antibacterial agents and DPPH/ABTS·+ radical scavengers, so they warrant further investigation.

【 授权许可】

CC BY   
© The Author(s). 2017