Effects of prostaglandin E1 on callus formation in rabbits

BMC Musculoskeletal Disorders

Research Article

Andreas J. Roth¹  Evgeny E. Krynetskiy²  Pawel V. Lipinsky³  Alexandr P. Oettinger³  Alexandr V. Skoroglyadov³  Alexandr B. But-Gusaim³  Ivan V. Sirotin³  Alexey V. Ivkov³ 

[1] Division for Total Joint Replacement/Orthopedics, Department of Orthopedics, University Hospital Leipzig, Traumatology and Plastic Surgery, Liebig Street 20, 04103, Leipzig, Germany;Joint Implant Surgeons of Florida 7331 College Pkwy, Suite #300, 33907, Fort Myers, FL, USA;Traumatology, Orthopaedics Department, 64 Clinical City Hospital, Russian State Medical University, Vavilova 61, 117292, Moscow, Russia;

关键词: PGE1;    Main Group;    Fracture Healing;    Callus Formation;    Intramedullary Nail;   

DOI  :  10.1186/s12891-015-0695-y

 received in 2015-02-02, accepted in 2015-08-24,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundRecent research has focused on identifying chemical modulators of osteogenesis. We present initial findings on the osteoinductive properties of prostaglandin Е1 (Vasaprostan), using a rabbit model.MethodsData were collected on callus formation in 14 male rabbits. These were divided into two groups (control and treatment) with 7 animals in each group. In all animals, the right tibia was fractured using a standardized protocol and stabilized by an intramedullary nail. Treatment group received a 5 μg/kg subcutaneous injection of PGE1/day during 10 postoperative days. Visual and radiological evaluation of callus formation was prospectively collected. After 30 days, all animals were killed and the tibia specimens were examined histologically.ResultsIn all the treatment group animals, fractures were consolidated radiologically by day 30. No treatment group animals and two control group animals were excluded form the experiment. In the control group, 4 animals demonstrated slower callus formation than the main group. Two control group animals were excluded from the experiment on the 20th day due to wound infection; one developed a nonunion.The mean coefficient of bone callus thickening in the main group was 2.08 (±0, 16) and 1.77 (±0.05) (p < 0.05) in the control group. Calculation of mean quantity of neogenic vessels in 10 random visual fields of the bone callus revealed 78 (±9.82) in the main group and 40 (±4.68) in the control group (p < 0.05).ConclusionsOur study demonstrates an increased rate and amount of bone callus formation in the group treated with prostaglandin E1 compared to the control group. Prospective radiological analysis was corroborated by histologic evaluation.

【 授权许可】

CC BY   
© Lipinsky et al. 2015